芦根提取液预防雄性大鼠草酸钙肾结石的研究  

Prevention of Extract from Rhizoma Phragmitis on Calcium Oxalate Stones in Male Rats

作　　者：贾希栋[1] 张春阳[2] 刘迎光[1] 张兵[3] 赵志博[3] 

机构地区：[1]辽宁医学院,辽宁锦州121000 [2]辽宁医学院附属第一医院泌尿外科,辽宁锦州121000 [3]吉林大学附属第三医院,长春130000

出　　处：《中国实验方剂学杂志》2013年第11期224-227,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅基金(2010276)

摘　　要：目的:研究芦根提取液对雄性大鼠肾草酸钙结石形成的影响。方法:将40只雄性SD大鼠随即分为5组,正常对照组(A)、模型组(B)、干预组(C,D,E),每组8只。采用1%乙二醇(EG)和1%氯化铵(AC)2 mL ig诱导大鼠肾草酸钙结石模型,每天1次,持续4周。造模同时C～E组每天分别给予剂量为1.0,1.5,2.0 g.kg-1的芦根提取物10 mL.kg-1ig。4周后检测大鼠24 h尿草酸(Ox),钙(Ca2+)浓度;血清尿素氮(BUN),肌酐(Cr),Ca2+,P含量;肾组织超氧化物岐化酶(SOD)的活性,丙二醛(MDA)含量;观察大鼠肾组织病理学改变和草酸钙结晶沉积情况;免疫组化检测大鼠肾组织骨桥蛋白(OPN)的表达水平。结果:模型组大鼠24 h OX,Ca2+浓度,血清Cr较正常对照组明显升高(P<0.05),干预组较模型组明显下降(P<0.05)。肾组织SOD的活力,模型组较正常对照组显著降低(P<0.05),干预组较模型组明显升高(P<0.05)。MDA含量,模型组较正常对照组明显降低(P<0.05),干预组较模型组明显降低(P<0.05)。免疫组化OPN在各组肾组织中均有表达,正常对照组中仅能检测到OPN的微弱表达,但模型组OPN表达则明显增强;干预组OPN表达强度低于模型组。结论:芦根提取液对雄性大鼠肾草酸钙结石的形成有抑制作用。Objective： To observe the influence of extract from Rhizoma Phragmitis on male rats renal calcium oxalate. Method： Forty male SD rats were randomly divided into five groups（n=8 each）：control, model, and Rhizoma Phragmitis intervention groups. After 4 weeks,the 24 h urine concentration oxalate（Ox）,Ca^2＋,the concentration of serum blood urea nitrogen（BUN）,creatinine（Cr）,Ca^2＋,P,and malondialdehyde（MDA）,superoxide dismutase（SOD） of renal tissue were detected. The calcium oxalate deposit in the kidney was observed by microscopy. Immunohistochemistry were used to assess the protein expression of osteopontin（OPN） in rat renal tissue of every groups. Result： The 24 h urine concentration Ox,Ca^2＋ and serum Cr of Rhizoma Phragmitis intervention group was lower than stone formation group obviously（P〈0.05）.rhizoma phragmitis intervention group the enzyme activity of SOD of renal tissue was higher than stone formation group（P〈0.05）. Stone formation group concentration of MDA of renal tissue was higher than Rhizoma Phragmitis intervention group（P〈0.05）.In normal control group, only a weak OPN protein staining was found. The expression was enhanced in the stone group. The extract from Rhizoma Phragmitis-treated rats tended to have lower OPN protein expression than the stone group. Conclusion： The extracts from rhizoma phragmitis can block the formation of renal calcium oxalate stone in rats potently.

关 键 词：芦根 草酸钙 肾结石 骨桥蛋白 

分 类 号：R285.5[医药卫生—中药学]