麝香乌龙丸对兔膝骨关节炎软骨组织形态及p38MAPK,Caspase-3表达的影响  

Effect of Shexiang Wulong Pill on Pathomorphology and Expression of p38 MAPK and Caspase-3 of Cartilage in Rabbits with KOA

作　　者：高润成[1] 王志文[1] 袁强[2] 张爱国[2] 杨雨旸[1] 李怡良[1] 项南[1] 

机构地区：[1]河北联合大学,河北唐山063000 [2]河北联合大学附属医院,河北唐山063000

出　　处：《中国实验方剂学杂志》2013年第11期228-231,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察兔膝骨关节炎(KOA)软骨组织形态及p38丝裂原活化蛋白激酶(p38 MAPK)、半胱氨酸天冬氨酸特异性蛋白酶3(Caspase-3)的表达,探讨麝香乌龙丸治疗兔KOA的作用机制。方法:将40只成年雄性大耳白兔,随机分为正常组(A)10只,造模组30只。采用关节腔内注射1.6%木瓜蛋白酶方法建立兔KOA模型,造模成功后随机分为模型组(B)、麝香乌龙丸低、高剂量组(C,D,n=10),ig给予麝香乌龙丸0.6,1.2 g.kg-1.d-1,灌胃4周后处死动物切取软骨组织,观察软骨组织形态变化,免疫组化染色检测关节软骨细胞中p38MAPK,Caspase-3的表达。结果:麝香乌龙丸低、高剂量组均能减轻兔软骨的病理损害,与模型组比较差异有统计学意义(P<0.05);麝香乌龙丸低、高剂量组均能降低软骨中p38MAPK及Caspase-3表达,与模型组比较具有显著差异(P<0.01)。结论:麝香乌龙丸能够减轻兔骨关节炎软骨病理损害;麝香乌龙丸治疗骨关节炎的机制,与其能降低p38MAPK,Caspase-3的表达,抑制软骨细胞过度凋亡有关。Objective： To study expression of p38 mitogen-activated proteikinase（p38 MAPK） and Caspase-3 in cartilage of the rabbit of knee osteoarthritis （KOA）,and discuss the possible mechanisms by Shexiang Wulong pill in treatment of osteoarthritis. Method： Forty only adult male rabbits were randomly divided into 4 group he control group,the model group, high dose group and low dose group of Shexiang Wulong pill （n=10）. 1.6% papain protein was injected to the rabbit knee joint cavity to establish rabbit knee osteoarthritis model, Shexiang Wulong pill groups were ig treated with Shexiang Wulong pill 0.6, 1.2 g·kg^-1·d^-1 for 4 weeks. To observe the pathomorphology of cartilage HE staining was used and the expression levels of p38MAPK and caspase-3 was detected by immunohistochemistry technology. Result： Shexiang Wulong pill could alleviate the rabbit cartilage pathological changes. Compared with the model group, Shexiang Wulong pill could reduce the expression of p38MAPK and Caspase-3 in cartilage tissue （P〈0.01）. Conclusion： The Shexiang Wulong pill may reduce expression of p38MAPK and Caspase-3,and inhibit excessive cartilage cell apoptosis-related.

关 键 词：麝香乌龙丸 骨关节炎 软骨 P38丝裂原活化蛋白激酶 半胱氨酸天冬氨酸特异性蛋白酶3 

分 类 号：R285.5[医药卫生—中药学]