清营解表合剂对流感病毒感染小鼠外周血Th1,Th2型细胞因子动态表达的影响  

Influence of Qingying Jiebiao Mixture on Dynamic Expression of Peripheral Blood Cytokines Th1,Th2 in Mice Infected by Influenza Virus

作　　者：任亮[1] 宋素花[1] 姜璐[1] 娄政驰[1] 宋荣强[1] 刘永[1] 吕翠霞[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2013年第11期255-260,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30672589)

摘　　要：目的:探讨清营解表合剂对流感病毒感染小鼠免疫功能的影响。方法:以流感病毒亚洲甲型鼠肺适应株(A/PR/8/H1N1)感染小鼠为模型,灌服清营解表合剂16 g.kg-1(0.8 g.mL-1,0.4 mL/只),1次/d,连续ig给药12 d,应用流式细胞仪分析药后3,6,9,12 d特异性细胞因子γ-干扰素,肿瘤坏死因子-α,白细胞介素3,4,6,10的动态表达水平及Th1(IFN-γ)/Th2(IL-4)值。结果:流感病毒感染小鼠后3 d,细胞因子IFN-γ升高显著(P<0.05),6,9,12 d无显著改变,6,9 d较3 d均降低显著(P<0.05);3,6,9,12 d细胞因子TNF-α均升高显著(P<0.01,P<0.05);3,6,9,12 d细胞因子IL-3均升高,3,6 d差异显著(P<0.05);3,6,9,12 d细胞因子IL-4,IL-6,IL-10均显著升高(P<0.01),在早期3 d达到峰值;3,6,9,12 d外周血Th1(IFN-γ)/Th2(IL-4)明显降低(P<0.01,P<0.05),且6 d降到最低点,12 d较6 d升高显著(P<0.05)。经清营解表合剂治疗后,3 d细胞因子IFN-γ水平升高,较模型组无显著差异,6,9,12 d较模型组升高显著(P<0.01,P<0.05);3,6,9,12 d细胞因子TNF-α水平表达较模型组均明显下降(P<0.01,P<0.05);3,6,9,12 d细胞因子IL-3较模型组均有所下降,但差异无统计学意义;3,6,9,12 d细胞因子IL-4,IL-6,IL-10水平较模型组均显著降低;3,6,9,12 d外周血Th1(IFN-γ)/Th2(IL-4)均升高,差异显著(P<0.01),12 d清营解表组较3 d升高显著(P<0.01)。结论:清营解表合剂通过调节流感病毒感染小鼠外周血Th型细胞因子的表达水平,恢复机体的抗感染免疫平衡,提高机体免疫功能,以防治流感病毒感染。Objective： To investigate the influence of Qingying Jiebiao mixture （QJM） on immune function in mice infected by flu virus. Method： The model of influenza virus in mice was established by lung infection with Asia line（A/PR/8/H1N1）. QJM suspension （0.8 g·mL^-1） 0.4 mL was orally given to each mouse （16 g·kg^-1） daily for 12 days. After administration of 3, 6, 9 d and 12 d, the expression level of specific cytokine interferon-gamma, tumor necrosis factor-α, interleukin 3, 4, 6, 10, the ratio of Th1 （interferin-γ, IFN-γ） and Th2 （interleukin-4, IL-4） were determined by flowcytometry. Result： After the mice was infected with influenza virus, cytokines IFN-γ at 3 d was increased significantly （P〈0.05）, those at 6, 9 d and 12 d showed no significant changes. IFN-γ at 6, 9 d was decreased significantly compared with that at 3 d （P〈0.05）; and cytokine tumar necrosis factoi-α（TNF-α） at 3, 6, 9, 12 d were increased significantly （P〈0.01, P〈0.05）; cytokines IL-3 at 3, 6, 9, 12 d was increased, those at 3, 6 d showed significant difference （P〈0.05）. Cytokines IL-4, IL-6 and IL-10 at 3, 6, 9, 12 d were significantly increased （P〈0.01）, and reached the peak in the early 3 d. Peripheral blood Th1 （IFN-γ）/Th2 （IL-4） at 3, 6, 9,12 d were decreased obviously （P〈0.01, P〈0.05）, and dropped to the lowest point at 6 d, and then increased significantly at 12 d compared with that at 6 d （P〈0.05）. After treatment of QJM, cytokines IFN-γ at 3 d was slightly increased, without significance to the model, however those at 6, 9, 12 d were increased significantly （P〈0.01, P〈0.05） compared to the model. Cytokines level of TNF-α expression at 3, 6, 9, 12 d were significantly decreased compared to those in the model group （P〈0.01, P〈0.05）. Cytokine IL-3 at 3, 6, 9, 12 d was slightly decreased, without significance to the model. Cytokines IL-4, IL-6, IL-10 at 3, 6, 9, 12 d were significantly lower than those in the model group. Perip

关 键 词：清营解表合剂 流感病毒 细胞因子 流式细胞术 

分 类 号：R285.5[医药卫生—中药学]