健脾活血解毒法拆方含药血清对人大肠癌细胞HCT-116细胞的增殖抑制作用及其机制研究  

Inhibition of Drug-containing Serum Decomposed Formulas of Jianpi Huoxue Jiedu Method on Prolifation of Human Colorectal Cancer Cell Line HCT-116 Cells

作　　者：丁宁[1] 杨宇飞[1] 刘羿男[2] 许云[1] 何斌[1] 

机构地区：[1]中国中医科学院西苑医院,北京100191 [2]北京大学医学部基础医学院,北京100191

出　　处：《中国实验方剂学杂志》2013年第11期272-275,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨健脾活血解毒法拆方含药血清对人结直肠癌细胞系HCT-116细胞的生长、细胞凋亡率、细胞凋亡相关基因和自噬基因表达的影响。方法:实验分为对照组、健脾组、活血组、解毒组、健脾活血组、健脾解毒组和健脾活血解毒组,分别采用酸性磷酸酶法(APA)、流式细胞术(FCM)和Western blot法检测各组含药血清对肿瘤细胞的生长、细胞凋亡率的变化、凋亡相关基因Caspase-3表达以及自噬相关基因LC3表达。结果:各组10%含药血清作用24 h后均能抑制HCT-116细胞增殖,但组间不具有明显差异。光镜下可见,健脾活血解毒法各组含药血清作用24 h后,细胞数量减少,且体积变小;细胞早期及中晚期凋亡率与对照组相比无显著性差异,但自噬相关蛋白LC3出现活性剪切条带。结论:健脾活血解毒法含药各拆方血清体外可抑制人结直肠癌HCT-116细胞增殖,机制可能与自噬作用通路密切相关。Objective： To investigate the impacts of the drug-containing serum of decomposed formulas based on Jianpi Huoxue Jiedu method on growth, rate of apoptosis, apoptosis related genes and autophagy gene expression t in human colorectal cancer cell line HCT-116 cells. Method： The experiment was divided into a control group, Jianpi group, Huoxue group, Jiedu group, Jianpi＋Huoxue group Jianpi＋Jiedu group and Jiapi＋Huoxue＋Jiedu group respectively, using the acid phosphatase enzymatic （APA）, flow cytometry （FCM） and Western blot to assay each drug-containing serum＇ treated tumor cell＇s growth, apoptosis rate changes, apoptosis-related genes Caspase-3 expression and autophagy LC3 gene expression. Result： After 10% drug-containing serum treated for 24 h, it showed inhibited proliferation of HCT-116 cells, however, no significant difference between the groups was found. The decrease in cell number and the shrinking in volume could be observed by light microscope after drug-containing serum treated for 24 h. Early and late apoptotic rate compared with the control group showed no significant difference, but autophagy related protein LC3 appeared active shear bands. Conclusion： The drug-containing serum of Jianpi Huoxue Jiedu method can inhibit human colorectal cancer cell line HCT-116 cells on proliferation in vitro. The mechanism may be closely related to the autophagy pathway.

关 键 词：结直肠癌 HCT-116细胞 含药血清 自噬 凋亡 

分 类 号：R285.5[医药卫生—中药学]