Galectin-7在人和鼠同种异体心脏移植物中的表达及其意义  

Role of the Galectin-7 expression in cardiac allografts in human and mouse

作　　者：雒真龙[1] 周鸿敏[1] 黄霞[2] 方静[1] 徐洪来[2] 高义[2] 杨超[2] 潘铁成[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院胸心外科,武汉市430030 [2]华中科技大学同济医学院附属同济医院器官移植研究所

出　　处：《实用医学杂志》2013年第10期1567-1569,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:30972794);教育部"973"项目(编号:2009CB522407)

摘　　要：目的:通过检测发生急性排斥反应的小鼠及人移植心脏的galectin-7表达,进而分析galectin-7在急排发生过程中的作用。方法:建立小鼠心脏移植模型,分为鼠急排组和鼠对照组。Western blot法和免疫组化法检测小鼠移植心脏galectin-7的表达情况。选择15例心脏移植急排患者(人急排组)和10例正常人(人对照组),免疫组化法检测心肌组织galectin-7的表达。结果:鼠急排组galectin-7表达明显高于鼠对照组(1.34±0.18,0.77±0.03,P<0.01)。人急排组心脏galectin-7表达明显高于人对照组,且galectin-7主要定位于人急排心脏的浸润淋巴细胞及血管内皮细胞。结论:Galectin-7与人和鼠同种异体心脏移植急性排斥反应有相关性。Objective To explore the function of galectin-7 in acute rejection through detecting the expression galectin-7 in cardiac allografts in human and mouse.Methods Mouse models of cardiac allografts and isografts were constructed.Western blot and Immunohistochemistry（IHC） were used for detecting galectin-7 expression in the grafts.IHC was used for detecting galectin-7 expression in myocardial tissues of 15 heart transplant patients with acute rejection and 10 normal individuals.Results The expression of galectin-7 in mouse allografts was significantly higher than that in isografts（1.34 ± 0.18,0.77 ± 0.03,P 0.01）.Galectin-7 mainly located in lymphocytes and vascular endothelial cells in human allografts.Conclusion Galectin-7 is related to the acute rejection in human and mouse cardiac allografts.

关 键 词：Galectin-7 急性排斥反应 心脏移植 人 小鼠 

分 类 号：R654.2[医药卫生—外科学]