HypoderminC基因DNA疫苗的构建及其免疫原性试验  

Construction and Immunogenicity of DNA Vaccine with Hypodermin C Gene of Hypoderma Lineatum

作　　者：刘春霞[1] 白红岩[2] 娜仁高娃[2] 呼和巴特尔[2] 王文龙[2] 

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018 [2]内蒙古农业大学兽医学院农业部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特010018

出　　处：《中国兽医杂志》2013年第1期13-15,共3页Chinese Journal of Veterinary Medicine

基　　金：内蒙古自然科学基金(2009BS0403)

摘　　要：本试验以Hypodermin C(HC)基因原核表达载体pETHC为模板,PCR扩增HC基因,克隆测序后,亚克隆到真核表达载体pcDNA3.1(+)中,构建了DNA疫苗pcDNA-HC。pcDNA-HC体外转染小鼠胎儿成纤维细胞后,间接免疫荧光技术检测目的基因在转染细胞中的表达。以DNA疫苗pcDNA-HC免疫小鼠,对小鼠进行体液免疫水平检测。结果表明,所构建的DNA疫苗能转染到小鼠胎儿成纤维细胞中并正确表达,免疫小鼠血清IgG滴度随免疫次数的增加而升高。The Hypodermin C（HC） gene of H. Lineatum was amplificated by PCR using pETHC as the tem-plate, cloned and sequenced, then subcloned into pcDNA3.1 （＋）. AS a result, the pcDNA-HC was successfully constructed. The pcDNA-HC was transfected into mouse fetal fibroblast cells in vitro, and the expression of target gene in transfected cells was detected by indirect immunofluorescence. The results showed that the pcDNA-HC was transfected into mouse fibroblast cells and HC gene was successfully expressed. The IgG titers in immunized mice sera showed a gradual upward trend with the increasing of the immunized times.

关 键 词：牛皮蝇蛆病 HypoderminC DNA疫苗 

分 类 号：S858.23[农业科学—临床兽医学]