亚硝化反硝化聚磷菌的筛选及生化特性分析  

Screening and Biochemical Characteristics of Nitrosation Denitrifying Polyphosphate-accumulating Organisms

作　　者：张立成[1] 李艳美[1] 袁雅姝[1] 吴春蓉[1] 刘君凤[1] 

机构地区：[1]沈阳建筑大学市政与环境工程学院,辽宁沈阳110168

出　　处：《中国给水排水》2013年第11期77-80,83,共5页China Water & Wastewater

基　　金：辽宁省教育厅资助项目(20060692);沈阳建筑大学省级重点实验室开放基金资助项目(HJ-200602);格平绿色助学行动辽宁环境科研123工程项目

摘　　要：为了研究稳定运行的连续流双污泥亚硝化反硝化除磷系统中微生物的种类和特性,利用混匀平板分离法、特殊染色法和一系列的生理生化试验对系统缺氧池内的微生物进行筛选和生化特性研究。试验分离出了7株亚硝化反硝化聚磷菌,分别属于葡萄球菌属、芽孢杆菌属、副球菌属、克雷伯氏菌属、泛菌属、肠杆菌属和莫拉氏菌属。通过特殊染色和吸磷试验发现,厌氧阶段亚硝化反硝化聚磷菌体内的聚磷颗粒逐渐减少,PHB颗粒明显增多;缺氧阶段PHB含量下降,生物体内聚合磷酸盐增加。缺氧反应20 h后,葡萄球菌属、芽孢杆菌属、副球菌属、克雷伯氏菌属、泛菌属、肠杆菌属和莫拉氏菌属的单位菌体吸磷量分别为1.98×10-11、1.64×10-11、1.43×10-11、1.13×10-11、9.59×10-12、7.72×10-12、6.28×10-12mg/CFU。In order to study the microbes and their characteristics in the continuous flow double sludge nitrosation denitrifying phosphorus removal system in steady state operation, the microbes in the anoxic reactor of the system were separated and identified through plate screening method, special staining and a series of physiological and biochemical tests. Seven nitrosation denitrifying phosphorus-accumulating organisms were separated, which were Staphylococcus, Bacillus, Paracoccus, Klebsiella, Pantoea, Enterobacteriaceae and Moraxella. By special staining and dephosphatation experiments, it was found that the phosphorus particles in nitrosation denitrifying phosphorus-accumulating organisms declined gradually and PHB particles increased apparently in the anaerobic stage. PHB declined and poly-phos phate increased in the anoxie stage. After 20 h of anoxic reaction, the quantities of phosphorus uptake of Staphylococcus, Bacillus, Paracoccus, Klebsiella, Pantoea, Enterobacteriaceae and Moraxella were 1.98 ×10^-11 mg/CFU, 1.64 ×10^-11mg/CFU, 1.43 ×10^-11 mg/CFU, 1.13 ×10^-11 mg/CFU, 9.59×10^-12 mg/CFU, 7.72 ×10^-12 mg/CFU and 6.28 ×10^-12 mg/CFU, respectively. Key words： double sludge system; nitrosation denitrifying phosphorus-accumulating organisms;

关 键 词：双污泥系统 亚硝化反硝化聚磷菌 生化特性 

分 类 号：X703[环境科学与工程—环境工程]