不同剂量γ射线照射人肝细胞株对BMPR2基因表达的影响研究  

Effect of γ-ray Irradiation With Different Dosage on the Expression of BMPR2 in Human Hepatocytes

作　　者：李建国[1] 尹晶晶[1] 袁慧[1] 秦秀军[1] 张伟[1] 闻建华[1] 

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,山西太原030006

出　　处：《中国辐射卫生》2013年第1期18-21,共4页Chinese Journal of Radiological Health

基　　金："十二五基金科研"资助项目项目编号(1201110202)

摘　　要：目的探讨人肝细胞株受不同剂量照射及照后不同时间点对骨形态蛋白受体2(BMPR2)的影响,试图为肝细胞的早期损伤提供依据。方法利用实时荧光定量PCR(Real–time PCR)技术对人肝细胞株受0.1、0.2、0.5、1、2、4 Gy不同剂量60Coγ射线照射及照后6、12、24 h点对BMPR2的表达情况进行研究。结果照后不同剂量点和不同时间点BMPR2基因和β-actin内参基因的溶解曲线均为单峰,说明均为特异性的扩增产物;照后6 h点BM-PR2基因不同剂量的相对定量比值均小于1,说明均为下调表达;照后12 h点BMPR2基因不同剂量的相对定量比值均大于1,说明均为上调表达;照后24 h点BMPR2基因除个别剂量点表现为下调表达趋势(0.2 Gy和2.0 Gy点),其余剂量点均表现为上调表达趋势。结论 BMPR2受不同剂量照射未表现出剂量效应关系,但随着照后时间不同,其表达趋势差异明显。Objective To investigate the effect of irradiation of different dosage and different time on the expression of bone morphogenetic protein receptor 2（BMPR2） in Human Hepatocytes,and provide evidence for early damage of hepatocyte.Methods The human hepatocytes were irradiated with 60Co γ-ray at 0.1,0.2,0.5,1,2 and 4 Gy,BMPR2 expression was detected by real–time fluorescent quantitative PCR after irradiation 6,12 and 24h.Results Melting curve of β-actin gene and BMPR2 with different dosage and time was the single peak,which means the specific ampliations.Relative quantitative ratio of different dosage irradiated 6 h lower than 1 showed down-regulated.Ratio of irradiated 12 h greater than 1 showed up-regulated.Irradiation 24 h,0.2 and 2.0 Gy showed down-regulated,and 0.1,0.5,1 and 4 Gy showed up-regulated.Conclusion BMPR2 does not show dose-effect relationship irradiated by different dosage,but shows obviously differential expression with different time.

关 键 词：Γ射线 人肝细胞 骨形态蛋白受体2(BMPR2) 实时荧光定量PCR 

分 类 号：Q691.8[生物学—生物物理学]