茶树硒营养代谢关键酶ATP硫化酶基因克隆及启动子结构分析  

The Gene Cloning of ATP Sulfurylase of Tea Piant,the Key Enzyme Related to Selenium Metabolism and the Structure Analysis of It’s Promoter

作　　者：王雅楠[1] 朱林[1] 叶爱华[2] 陶少强[1] 秦冰[1] 

机构地区：[1]安徽农业大学资源与环境学院,安徽合肥230036 [2]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《热带作物学报》2013年第4期654-661,共8页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金"茶树硒营养代谢关键酶基因的表达及其调控机理研究"(No.30971870)

摘　　要：为探明茶树硒营养代谢关键酶基因ATP硫化酶的表达调控规律,通过PCR获得APS的DNA全长序列,应用genome walking克隆了APS基因的5′侧翼序列,采用PLACE、PlantCARE在线启动子预测工具分析表明:该序列含有启动子的特定结构,如TATA-box,CAAT-box等,为深入研究茶树硒营养代谢关键酶的表达调控机制奠定了理论基础。In order to explore the regulation and control of the gene expression of ATP sulfurylase（APS）,the key enzyme related to selenium metabolism,we cloned the APS DNA fragments from tea plant by PCR,and cloned the 5′ flanking sequences of APS genes by application of the genome walking technology.Using the online promoter prediction tool of PLACE,Plant CARE to analysis the gene structure,the results showed that the gene sequence contains the specific structures of a promoter,such as TATA-box,CAAT-box,etc.The results established a certain theoretical foundation for the expression of the key enzyme gene related to selenium metabolism.

关 键 词：茶树 基因克隆 ATP硫化酶 启动子 基因组步移 

分 类 号：S571[农业科学—作物学] Q523[生物学—生物化学]