猪釉基质蛋白对MC3T3-E1成骨细胞增殖分化的影响  

Influences of Porcine Enamel Matrix Proteins on MC3T3-E1 Osteoblast Proliferation and Diffentiation

作　　者：束蓉[1] 刘正[1] 葛琳华[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔内科,200011

出　　处：《华西口腔医学杂志》2000年第4期226-228,共3页West China Journal of Stomatology

摘　　要：目的 :探索釉基质蛋白 (EMPs)促进成骨细胞增殖分化的作用 ,为进一步研究提供基本数据。方法 :选择MC3T3 E1成骨细胞株 ,利用体外细胞培养方法 ,分别加入不同浓度的EMPsα MEM培养液 ,于培养 0、1、2、3、4,5、6d进行细胞计数 ,采用SAS软件对细胞计数数据作单因素方差分析。结果 :EMPs各组细胞计数均高于阴性对照 (C)组 (P <0 0 1)。第 2天 ,10 0 μg/ml和 15 0 μg/mlEMPs组的细胞计数高于阳性对照 (T)组 (P <0 0 1) ;第 5天 ,10 0μg/mlEMPs组的细胞计数不但高于C、T组 ,还明显高于其它EMPs浓度组。结论 :釉基质蛋白可明显促进成骨细胞的增殖分化。Objective:Some research suggested that enamel matrix proteins(EMPs) not only induced formation of cementum, but also could promote regeneration of periodontal tissue and alveolar bone. In order to recognize the mechanisms of periodontal regeneration induced by EMPs and provide a basic data for further study, influences of EMPs on osteoblast proliferation and differentiation are explored in this study.Methods:Porcine enamel matrix proteins were extracted by using acetic acid. MC3T3 E1 osteoblasts were selected and cell culture was used in vitro. EMPs with different concentrations of 50μg/ml, 100μg/ml and 150μg/ml were added separately in α MEM culture medium and compared with negative group and positive group (TGF β\-1). The count of cell was recorded after 0,1,2,3,4,5,6 days, then the data were analysed satistically by using SAS.Results: The cell count of all EMPs groups were higher than that in the negative control group (P<0 01), 100μg/ml as well as 150μg/ml EMPs groups were higher than the positive group(TGF β\-1) on the second day (P<0 01). The cell count of 100μg/ml EMPs group was not only higher than C and T groups, but also higher than that of other EMPs dose groups on the fifth day.Conclusion: The proliferation and differentiation of osteoblasts can be promoted distinctly by enamel matrix proteins.\;

关 键 词：猪釉基质蛋白 MC3T3-E1 成骨细胞 牙槽骨再生 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]