解淀粉芽孢杆菌Lx-11产脂肽类物质鉴定及表面活性素对水稻细菌性条斑病的防治作用  

Identification of the Lipopeptides from Bacillus amyloliquefaciens Lx-11 and Biocontrol Efficacy of Surfactin Against Bacterial Leaf Streak

作　　者：张荣胜[1] 王晓宇[1] 罗楚平[1] 刘永锋[1] 刘邮洲[1] 陈志谊[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《中国农业科学》2013年第10期2014-2021,共8页Scientia Agricultura Sinica

基　　金：国家公益性行业(农业)科研专项经费项目(201103002-3;201303015);江苏省农业科技自主创新资金项目(CX(11)2045)

摘　　要：【目的】对解淀粉芽孢杆菌Lx-11产生的脂肽类物质进行分离鉴定和水稻条斑病防效测定。【方法】根据脂肽类抗菌物质合成相关基因序列对Lx-11进行PCR扩增检测,采用MALDI-TOF-MS对脂肽类物质进行分析。【结果】通过3对特异性引物对Lx-11菌株检测,扩增产物经克隆、测序和BLAST分析,表明解淀粉芽孢杆菌Lx-11基因组中含有sfp、fenB、ituA或bamA基因。质谱分析发现提取的粗提物中含有surfactin、bacillomycin D和fengycin等3种脂肽类抗生素。通过构建surfactin突变体菌株,平板和盆栽试验显示突变体菌株抑制条斑病菌能力显著降低。【结论】解淀粉芽孢杆菌Lx-11产生的surfactin类物质对条斑病菌具有较强的抑制作用,是抑制条斑病菌主要物质之一。【Objective】The objective of this study is to isolate and identify the lipopeptides produced by Bacillus amyloliquefaciens Lx-11 and their biocontrol efficacy against bacterial leaf streak（BLS）.【Method】 Three primers designed based on the known lipopeptide genes were used to amplify the corresponding genes from the genome of Lx-11 strain.Lipopeptides were identified by MALDI-TOF-MS techniques.【Result】The PCR products with three primer pairs were cloned and sequenced.The BLAST analysis showed that the sfp,fenB,ituA or bamA genes exist in the genome of B.amyloliquefaciens Lx-11.Three kinds of lipopeptides surfactin,bacillomycin D and fengycin were identified in the antimicrobial extract by the mass spectrum analysis.The antibacterial activity could be associated with surfactin-lipopeptides,and practically abolished in surfactin-deficient mutants.Lx-11 strain significantly reduced disease incidence of BLS.【Conclusion】The antibacterial activity of the surfactin-deficient mutant strain was practically abolished,which suggested that surfactin might play an important role in control of BLS.

关 键 词：解淀粉芽孢杆菌 细菌性条斑病 脂肽类物质 生物防治 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]