羊驼毛纤维中黑色素含量的相关性研究  

The Correlationship Between Different Kinds of Melanins in the Fleece of Alpaca(Lama Pacos)

作　　者：范瑞文[1] 杨姗姗[1] 白俊明[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国农学通报》2013年第14期7-10,共4页Chinese Agricultural Science Bulletin

基　　金：山西省科技攻关项目"利用羊驼CDK5基因改变绵羊毛色"(20120311034-2);国家自然科学基金"羊驼酪氨酸酶相关蛋白1基因及其功能的研究"(30671512)

摘　　要：为研究羊驼毛纤维中黑色素含量的相关性,以为建立鉴定羊驼毛色的参数标准提供理论依据。选择肉眼观察认为表型不同的22种自然毛色的羊驼,每个表型选取3只羊驼,从背部采集羊驼毛,用不同的方法溶解,以乌贼黑为标准品,用紫外分光光度计法测定羊驼毛纤维中碱性可溶总黑色素(ASM)、真黑素(EM)和褐黑素(PM)的含量,通过单因素方差和线性回归曲线分别分析不同毛色羊驼毛纤维中黑色素的差异性和相关性。结果表明:ASM和EM在不同毛色的毛纤维中呈差异显著(分别为P<0.01和P<0.05),而PM呈差异不显著(P>0.05)。线性回归曲线得到模式方程式:模式1:Sp.ASM=-0.313+3.168×Sp.EM+E(R2:0.902,t=-3.732,P=0.000,E为误差项;模式2:Sp.EM=0.285+0.143×Sp.ASM+E(R2:0.902,t=6.769,P=0.000,E为误差项。因此,羊驼ASM和EM与毛色具有很大的相关性,可以作为区别毛色的重要参数,二者间存在线性回归。To supply the evidence of identification of alpaca hair color, the difference and correlation between different melanins in alpaca hair was studied. According to the visual inspection, 22 different hair color genotypes with 3 alpacas each were selected. Alpaca fleece was taken from the back and then dissolved with different solution to get melanins. The Amount of Total Melanins （ASM）, Eumelanin （EM） and Pheomelanin （PM） in alpaca fleece with different hair color were measured with spectrophotometric assay, sepia as a standard for the spectrophotometric assay. Then the difference and correlation between the amount of melanins were analyzed by one-way ANOVA and linear regression curve method, respectively. The results showed that there were difference in ASM （P〈0.01） and EM （P〈0.05） and no difference in PM （P〉0.05） between alpaca fleece with different hair color, and that two models were deduced from the linear regression curve analysis： model 1： Sp.ASM=-0.313＋3.168xSp.EM＋E（R2：0.902, t=-3.732, P=-0.000）; model 2： Sp.EM=0.285＋0.143x Sp.ASM＋E（R2：0.902,t=6.769,P=-0.000）. It was suggested that ASM and EM could be used as the parameters to differentiate hair color of alpaca because of significant correlation to hair color, moreover, both of them have the relation of linear regression curve.

关 键 词：碱性可溶总黑色素(ASM) 真黑素(EM) 褐黑素(PM) 羊驼毛纤维 毛色 

分 类 号：S829.9[农业科学—畜牧学]