用双波长分光光度法测定大鼠小肠循环液中酚红的含量  

Determination of the Content Phenol Red in the Rat′s Small Intestine Circulation Solution by Dual Wavelength Spectrophotometry

作　　者：吴刚[1] 何勇[1] 王红梅[2] 

机构地区：[1]佳木斯大学药学系,佳木斯154003 [2]佳木斯市中心医院

出　　处：《沈阳药科大学学报》2000年第5期341-342,共2页Journal of Shenyang Pharmaceutical University

摘　　要：采用双波长分光光度法测定了大鼠小肠循环液中酚红的含量 ,方法的线性范围为 1～ 8mg/L ,回收率为 99 88%～ 99 99% (n =5 ) ,日内RSD为 0 5 1%～ 1 2 7% (n =4) ,日间RSD为0 5 8%～ 2 2 9% (n =4) .A spectrophotometric method has been developed for the determination of phenol red in rat′s small intestine circulation solution. Dual wavelength spectrophotometry was used at 558 nm and 570 nm. 0 1 mol/L NaOH was selected as the solvent.The calibration curve was linear in the range from 1 to 8 mg/L . The recoveries were 99 88%～99 99%. The within day precision was 0 51%～1 27% and the day to day precision was 0 58%～2 29%. This method can be used to determine phenol red in rat′s small intestine circulation.

关 键 词：酚红 双波长分光光度法 小肠循环液 大鼠 

分 类 号：R969.1[医药卫生—药理学]