两种杨树树皮内生真菌多样性及优势种群动态变化  

Predominant Species Dynamic and Diversity of Fungal Endophytes in Barks of Two Populus Cultivars

作　　者：李永[1] 朴春根[1] 郭利民 常聚普[2] 王海明 贺伟[4] 谢守江 郭民伟[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所,北京100091 [2]濮阳市林业科学院,河南濮阳457000 [3]菏泽市林木保护站,山东菏泽274002 [4]北京林业大学林学院,北京100083

出　　处：《林业科学研究》2013年第3期292-298,共7页Forest Research

基　　金：森林生态环境与保护研究所所基金(CAFRIFEEP201102-4);林业公益性行业专项(201104054);国家微生物资源平台:林业微生物库藏资源管理与共享(NIMR-2011-7)

摘　　要：为了解健杨94(转基因抗虫杨94)、三倍体毛白杨2个杨树品种干部树皮内生真菌区系及其优势种群的季节变化情况,本研究利用组织分离法从2个杨树品种996块组织中分离内生真菌1 175株,健杨94和三倍体毛白杨分别分离612、563株。利用形态特征和分子生物学方法鉴定为15个属、35个分类单元,包括担子菌1个分类单元,子囊菌34个分类单元。2个杨树品种内生真菌优势种群包括链格孢、葡萄座腔菌、镰孢属真菌、间座壳属真菌等,其中,仅有链格孢、葡萄座腔菌和桑砖红镰孢是两品种共有的优势种群种类,而且优势种群会随季节变化而变化。在两品种的内生真菌中,链格孢、葡萄座腔菌是最为常见的优势种群。In order to understand the predominant species dynamic and diversity of fungal endophytes in barks of Populus x euramericana cv. Robusta 94 and triploid of P. tomentosa, the fungal endophytes were isolated from barks of P. x euramericana cv. Robusta 94 and triploid of P. tomentosa by tissue isolation, and identified based on sequence analysis of the internal transcribed spacer and morphological characterization by microscopic observations. A total of 1 175 fungal endophytes were isolated from 996 bark tissues. The fungal endophytes were classified into 35 fungal taxa belonging to 15 genera, including 1 species of Basidiomycota and 34 taxa of Ascomycota. And Alternaria alternata, Botryosphaeria dothidea, Fusarium spp. , and Diaporthe conorum were the predominant species of fungal endophytes in barks of P. × euramericana cv. Robusta 94 and triploid of P. tomentosa, while A. alternata and B. dothidea were the most common predominant species in the barks of the two cuhivars. The variation of predominant fungal endophytes of two poplar species in different seasons was detected.

关 键 词：内生真菌 优势种群 多样性 树皮 

分 类 号：S718.8[农业科学—林学]