毛竹花期不同器官内源激素含量的变化  

Changes of Endogenous Hormones in Different Organs during the Flowering Phase of Moso Bamboo

作　　者：齐飞艳 彭镇华 胡陶 高健 

机构地区：[1]国际竹藤中心,竹藤科学与技术重点实验室,北京100102

出　　处：《林业科学研究》2013年第3期332-336,共5页Forest Research

基　　金："十二五"国家科技支撑计划课题"竹资源优良品种选择与育种技术研究"(2006BAD19B0203);国家林业公益性行业科研专项"毛竹基因组测序研究"(200704001)

摘　　要：用酶联免疫法测定未开花毛竹和开花毛竹叶、花、枝、秆和鞭中内源激素吲哚乙酸(IAA)、赤霉素(GA3)、脱落酸(ABA)和玉米素核苷(ZR)的含量,分析开花毛竹内源激素分布和含量的变化与花形态分化的关系。结果表明:与未开花毛竹相比,成花期(6月中旬)开花毛竹叶、枝和秆中的IAA含量显著降低,枝的ABA含量也显著降低,而秆的ZR含量显著升高;扬花期(7月中旬)开花毛竹枝中的IAA含量显著降低,而GA3含量则显著升高。内源激素在开花毛竹各器官中的分布规律:IAA为秆>鞭>枝>花,GA3为花>鞭>枝>秆,ZR为花>枝>秆>鞭,ABA为秆>枝>花>鞭。二级枝中IAA含量显著低于一级枝,而其GA3和ZR的含量高于一级枝。IAA、GA3和ABA在竹杆中的含量呈向基性增加,而ZR则呈向基性减少。在成花期和扬花期ABA、ZR和GA3所占比例高于未开花毛竹,高比例的ABA、ZR和GA3和低比例的IAA可能促进毛竹花的发育。Endogenous hormone contents of indole-3-acetic acid （IAA）, gibberellin A3 （GA3 ）, abscisic acid （ABA） and zeatin riboside （ZR）were measured by enzyme-linked immunosorbent assay （ELISA）in leaves, inflorescence, branches, stems and rhizomes of flowering and non-flowering moso bamboo （Phyllostachys edulis）, for discussing the differences and understanding the relation between endogenous hormones distribution and contents and flower＇ s morphological differentiation. The results showed that： in comparison with non-flowering moso bamboo, the content of IAA in the leaves, branches and stems of flowering bamboo in Heading stage （mid-June） reduced significantly, and the content of ABA in the branches brought down significantly too, but the content of ZR in stems increased significantly. The content of IAA in the branches of flowering bamboo in flowering period （mid-July）was lower significantly and the content of GA3 rose significantly. The distribution characters of hormone in different or- gans were found different, while that of IAA was stem 〉 rhizome 〉 branch 〉 inflorescence, that of GA3 was inflorescence 〉 rhizome 〉 branch 〉 stem, that of ZR was inflorescence 〉 branch 〉 stem 〉 rhizome and that of ABA was stem 〉 branch 〉 inflorescence 〉 rhizome. The content of IAA in secondary side branches was significantly lower than that in primary side branches, but the contents of GA3 and ZR were higher than that in the primary side branches. The contents of IAA, GA3 and ABA in bamboo stem increased basipetally, while that of ZR in stem decreased basipetally. High rates of ABA, ZR and GA3 and low proportion of IAA may be helpful to the flower development during flower development, because ABA, ZR and GA3 had higher proportion in Heading stage and flowering period.

关 键 词：形态分化 内源激素 开花 毛竹 

分 类 号：S795.7[农业科学—林木遗传育种]