林蛙油对游泳训练小鼠腓肠肌保护作用研究  

The Protective Effect of Rana Oil on Swimming Trained Mice Gastrocnemius

作　　者：张平[1] 张岚[2] 鲍九枝[1] 李松哲[1] 

机构地区：[1]吉林医药学院军事体育教学部,吉林吉林132013 [2]吉林医药学院营养与食品卫生教研室,吉林吉林132013

出　　处：《食品研究与开发》2013年第7期16-19,共4页Food Research and Development

基　　金：吉林省教育厅"十二五"科学技术研究重点课题(吉教科合字[2011]第282号)

摘　　要：探讨林蛙油对运动训练小鼠腓肠肌自由基代谢和超微结构的影响,为林蛙油的应用和开发提供实验依据。以ICR小鼠为研究对象,通过建立灌服林蛙油小鼠递增游泳训练和游泳力竭模型,测定小鼠力竭游泳时间、腓肠肌SOD活性、MDA含量和小鼠腓肠肌超微结构的变化。林蛙油能延长小鼠力竭游泳时间,与运动组相比,林蛙油中、高剂量组有显著差异(p<0.01)。运动组SOD活性显著降低,与安静组比较,有显著差异(p<0.01);与林蛙油各剂量组比较,中、高剂量组有显著差异(p<0.05)。运动组MDA的含量最高,与安静组比较,有显著异(p<0.01)与林蛙油各剂量组比较,均有显著异(p<0.01)。小鼠腓肠肌组织超微结构的观察表明,运动组肌原纤维上肌节结构可辨,但呈致密状,线粒体嵴断裂,呈空化状,林蛙油组损伤程度小于运动组。林蛙油可显著提高小鼠腓肠肌组织抗自由基氧化的功能,对运动造成的腓肠肌组织超微结构的损伤有明显的保护作用,对小鼠运动能力的提高有很好的作用。To explore the impact of Rana oil on trained mice Gastrocnemius free radical metabolism and uhrastructure, provided the experimental basis for the application and development of Rana oil. The research aimed to establish the oil Rana mice fed the incremental swimming training and exhaustive swimming model. Changes of mice swimming exhausted time, gastrocnemius muscle SOD activity, MDA content and gastrocnemius muscle uhrastructure was measured. The Rana oil can extend the swimming exhausted time of mice, compared with the trained group, Rana oil of high and medium dose group had a significant difference （p 〈0.01 ）. SOD activity in the trained group significantly reduced, compared with the quiet group, had a significant difference （p 〈0.01 ） ; comparison with Rana oil each dose group, the high and medium dose group had a significant difference （p 〈0.05）. The highest MDA content was in trained group, compared with quiet group was significantly different （p 〈0.01 ） ; with Rana oil each dose group were significantly different （p 〈 0.01）. The gastrocnemius muscle ultrastructure observation showed that the sarcomere structure on the myofibrillar of the trained group is legible, but was dense, mitochondrial cristae broken, was cavitation-like, the Rana oil group degree of injury is less than the trained group. Rana oil can significantly improve the function of anti-free radical oxidation of mouse Gastrocnemius tissue, had a significant protective effect against ultrastructural damage of the Gastrocnemius tissue caused by the movement, had a good role to improve exercise capacity in mice.

关 键 词：林蛙油 超氧化物岐化酶(SOD) 丙二醛(MDA) 腓肠肌 超微结构 

分 类 号：R285.5[医药卫生—中药学] G861.1[医药卫生—中医学]