桤叶唐棣组培技术  

A tissue culture technique for Amelanchier alnifolia

作　　者：王占龙[1] 黄立华[1] 龙忠伟[1] 

机构地区：[1]辽宁省干旱地区造林研究所,辽宁朝阳122000

出　　处：《林业科技开发》2013年第3期123-125,共3页China Forestry Science and Technology

基　　金：国家林业局"948"项目"桤叶唐棣种质资源及果实加工技术引进"(编号:99-4-08)

摘　　要：对桤叶唐棣进行组织培养研究,结果表明,诱导培养基配方为MS+BA 0.6mg/L+NAA 0.12mg/L+IBA0.4mg/L+蔗糖35g/L+琼脂8g/L,诱导率达到了74%;增殖培养基配方为MS+BA 1.5mg/L+IBA 1.0mg/L+蔗糖35g/L+琼脂8g/L,增殖倍数达到5.56倍;生根培养基配方为1/2MS+NAA 0.05mg/L+IBA 1.0mg/L+蔗糖35g/L+琼脂8g/L,生根率达91%。A research on tissue culture of Amelanchier alnifolia was summarized and the optimal mediums were proposed in this paper. The medium of MS ＋ BA 0.6 mg/L ＋ NAA 0.12 mg/L ＋ IBA 0.4 mg/L ＋ sucrose 35 g/L ＋ agar 8 g/L was op- timal for inducement, the inducement rate could be up to 74% ; The medium of MS ＋ BA 1.5 mg/L ＋ IBA 1.0 mg/L ＋ su- crose 35 g/L ＋ agar 8 g/L was optimal for subculture, and the number of proliferation was 5.56 ; The medium of 1/2MS ＋ NAA0.05 mg/L ＋ IBA 1.0 mg/L ＋ sucrose 35 g/L ＋ agar 8 gilL was optimal for rooting, and the rooting rate was 91%.

关 键 词：桤叶唐棣 组织培养 工厂化育苗 

分 类 号：S723.13[农业科学—林木遗传育种]