孝感市儿童红细胞锌浓度的测定及其正常参考范围  

Erythrocyte Zinc Concentration Determination of Xiaogan Area Children and Normal Reference Range

作　　者：刘自刚[1] 王少敏[1] 陈早立[1] 徐凤兰[1] 

机构地区：[1]孝感市中心医院,湖北孝感432000

出　　处：《广东微量元素科学》2013年第2期11-15,共5页Trace Elements Science

基　　金：2011年湖北省自然科学基金项目(项目编号:2011CDB324)

摘　　要：目的运用原子吸收分光光度法测定红细胞锌浓度并建立起本地区儿童红细胞锌浓度正常参考范围。方法建立起红细胞锌的测定方法并进行方法学评价,同时通过对本地区健康儿童红细胞锌的测定,建立起本地区儿童红细胞锌浓度正常参考范围。结果原子吸收光谱法测定红细胞锌浓度的线性范围广(33.4～400.5μmol.L-1),准确度高(回收率分别为:93.5%,96.2%,104.7%),精密度好(批内CV分别为2.49%、2.02%,批间CV分别为3.65%、2.93%),抗干扰能力强(携带污染率:0.55%,干扰实验结果:1.93%、1.85%、1.62%)。不同年龄段儿童的红细胞锌浓度有一定的差异,男女性别上没有差异。结论所建立测定红细胞锌浓度的方法适合在临床实验室应用,本地区不同年龄段儿童红细胞锌浓度的正常参考范围可能具有不同的临床意义。Objective Using atomic absorption spectrophotometry erythrocyte zinc concentration and set up the region children erythrocyte zinc concentration normal reference range. Method The measurement method of erythroeyte zinc was established the evaluation of methodology was done, and at the same time, through this area healthy children red blood cells of zinc determination, establish the region children erythrocyte zinc concentration normal reference range. Results By determing of this method, erythrocyte zinc concentration of linear range （ 33.4 - 400.5 μmol·L^-1 ） , high accuracy （ recovery rate were 93.5% , 96. 2% , 104. 7% ） , precision （ CV in group was 2.49% and 2. 02% , respectively, between batch CV were 3.65% , 2.93% ）, anti- interference ability （carry pollution rate： 0. 55% , interference experiment results： 1.93% , 1.85% , 1.62% ）. Different age paragraph the children erythrocyte zincconcentration have certain differences, there is no difference between male and female. established determination method to determine the concentration of zinc red blood application in laboratory, concentration of the normal the establishment reference range may of regional different have different clinical age children significance. Conclusion The cells for clinical erythrocyte zinc

关 键 词：儿童 红细胞锌 孝感市 

分 类 号：R446.112[医药卫生—诊断学]