神经肽ABNP的提取及其作用研究  

Isolation of ABNP and its anti morphine activity in mice

作　　者：安玉会[1] 高建松[2] 贺付成[1] 

机构地区：[1]河南医科大学,郑州450052 [2]郑州牧业工程高等专科学校,郑州450008

出　　处：《郑州牧业工程高等专科学校学报》2000年第3期161-163,共3页Journal of Zhengzhou College of Animal Husbandry Engineering

基　　金：河南省"九五"重大攻关课题! (9712 0 0 6 0 0 )

摘　　要：首次在牛脑中发现了一种新的酸性牛神经肽。用 Sephadex G5 0及硅胶柱层析对其进行了纯化 ,得率为 5‰。氨基酸分析及测序表明 ,其结构为 Glu- Glu- Glu。对小鼠的试验它有显著的抗吗啡作用 ,而无依赖性。Acid bovine neuropeptide(ABNP) was firstly found in the bovine brain. It was purified by Sephadex G50/Silica column chromatography. ABNP is a tripeptide, Glu Glu Glu, identified by National 835-50 Model amino acid analyzer and PE ABI 491A Model protein sequencer. It has significant anti morphine activity in mice but no addiction at doses 0.125～05g/kg.

关 键 词：牛神经肽 氨基酸分析 测序 依赖性 

分 类 号：Q516[生物学—生物化学] Q42