东北地区三种典型土壤磷组分的^(31)P核磁共振研究及其与土壤磷酸酶活性的关系  

Soil Phosphorus Composition by ^(31)P NMR Spectroscopy and Soil Phosphatase Activities of Three Typical Soils in Northeast China

作　　者：张广娜[1,2] 陈振华[1] 陈利军[1] 武志杰[1] 魏锴[1,2] 张艾明[1,2] 张玉兰[1] 

机构地区：[1]森林与土壤生态国家重点实验室中国科学院沈阳应用生态研究所,辽宁沈阳110016 [2]中国科学院研究生院,北京100049

出　　处：《土壤通报》2013年第3期617-623,共7页Chinese Journal of Soil Science

基　　金：国家自然科学基金项目(41171241);国家"十二五"科技支撑计划项目(2012BAD14B04)资助

摘　　要：应用液体31P核磁共振(31P NMR)技术对东北地区三种典型土壤--棕壤、黑土和褐土的磷组分进行了研究,并探讨了其与土壤磷酸单酯酶和二酯酶活性的关系。结果发现:棕壤和黑土中正磷酸盐和磷酸单酯各自约占土壤全磷的一半,褐土中主要的磷组分为正磷酸盐,单酯仅占总磷的18%;棕壤和黑土中焦磷酸盐含量较高,褐土中未鉴定到焦磷酸盐;褐土的石灰性性质是导致其正磷酸盐占主导地位、单酯含量少且没有发现焦磷酸盐的原因。三种土壤均鉴定出含有肌醇六磷酸盐(植酸盐),其中棕壤和褐土含有myo-植酸盐和一种未知植酸盐而黑土则含有scyllo-植酸盐。土壤磷酸酶活性的结果表明,黑土磷酸单酯酶活性和二酯酶活性均高于棕壤和褐土。土壤中正磷酸盐及磷酸单酯含量与土壤磷酸单酯酶和二酯酶呈极显著正相关关系。Soil phosphorus （P） composition of brown earth, black soil and cinnamon soil in Northeast China were studied by solution 31p nuclear magnetic resonance （np NMR） spectroscopy. Relationships between 3T NMR P composition and soil phosphatase activities were also discussed in this study. Results showed that orthophosphate and phosphate monoesters were accounted for half of soil phosphorus in brown earth and black soil, respectively. Orthophosphate was the dominant form of P in cinnamon soil while orthophosphate monoesters could only account for 18% of total P. Brown earth and black soil contained quantitative pyrophosphate while cinnamon soil had no pyrophosphate. Calcareous soil property of cinnamon soil caused its large amount of orthophosphate, less monoesters and no pyrophosphate in soil. Inositol phosphates were identified in the three soils. Brown earth and cinnamon soil contained myo-inositol phosphate and one unidentified inositol phosphate while black soil contained scyllo-inositol phosphate. Soil phosphatase showed that the activities in black soil were higher than those in brown earth and cinnamon soil. Soil orthophosphate and monoester contents all showed significantly positive relationships with soil phosphatase activities.

关 键 词：磷酸单酯 植酸盐 焦磷酸盐 棕壤 黑土 褐土 核磁共振 

分 类 号：S158.5[农业科学—土壤学]