新疆地区牛乳源金黄色葡萄球菌粘附因子ClfA基因的克隆与序列分析  

Clone and Sequence Analysis of ClfA Gene of Staphylococcus aureus Isolated from Bovine Milk in Xinjiang Area

作　　者：邵俊高[1] 苏艳[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《新疆农业大学学报》2013年第2期103-107,共5页Journal of Xinjiang Agricultural University

基　　金：新疆维吾尔自治区高新技术项目(201311108);国家自然科学基金项目(31160191;31260222);新疆维吾尔自治区普通高校重点学科基础兽医学科项目

摘　　要：采集新疆奶牛隐性乳腺炎乳样,对分离鉴定的致病性金黄色葡萄球菌,提取分离菌株的基因组DNA,参考GenBank中发表的ClfA(Clumping Factor A of Staphylococcus aureus)基因序列,设计特异性引物经PCR扩增得到1 560bp的ClfA基因。对新疆3个地区分离的9株金黄色葡萄球菌分别进行ClfA基因的克隆和序列分析,对ClfA蛋白的氨基酸序列分析结果表明,新疆3个地区ClfA蛋白氨基酸的同源性为91.5%～100%,与国外其他分离菌株的同源性为91.9%～100%,差异性较小,该结果表明ClfA基因在该菌进化和流行过程中是保守的。Staphylococcus aureus which caused subclinical mastitis in bovine was isolated from Xinjiang bovine milk and identified.According to the ClfA gene sequences published by GenBank,a pair of specific primers were designed.ClfA gene was amplified by PCR with genomic DNA and the CIfA gene fragment was 1560 bp. This gene fragment was cloned and gene was analyzed by sequencing. Our result showed that the amino acid homology of CIfA protein for 3 regions in Xinjiang area was 91.5%-100%,compared with other countris the amino acid homology of ClfA protein was 91.9%-100%. These data indicated that the ClfA gene was conserved during the bacterial evolution and prevalence and provided experiment foundation for using ClfA to prevent infection caused by S. aureus in future.

关 键 词：新疆 金黄色葡萄球菌 ClfA基因 克隆 序列分析 

分 类 号：S851.611[农业科学—预防兽医学]