基于分子瓦自组装的DNA纳米管的制备与表征  

Preparation and characterization of DNA nanotube based on DNA tile self-assembly nanotechnology

作　　者：叶日安[1] 周明[1,2] 庄立洲[1] AMOAKO George 杨晓红[1] 沈智勇[1] 

机构地区：[1]江苏大学光子制造科学技术中心,镇江212013 [2]清华大学摩擦学国家重点实验室,北京100084

出　　处：《科学通报》2013年第13期1248-1254,共7页Chinese Science Bulletin

基　　金：国家重点基础研究发展计划(2011CB013004);清华大学摩擦学国家重点实验室自主研究重点项目(SKLT10A02)资助

摘　　要：利用DNA分子自组装技术可以构建从一维到三维不同形状的纳米结构,并且这些结构在微纳米电子学、纳米生物学等众多领域有许多潜在的用途.本文利用DNA分子瓦(tile)自组装技术,采用双交叉(DX)DNA分子瓦成功组装了一维DNA纳米管结构,聚丙烯酰胺凝胶电泳(native-PAGE)、透射电子显微镜(TEM)、荧光显微镜和原子力显微镜(AFM)对制得的DNA纳米管结构进行了表征,结果表明,组装成功的DNA纳米管直径在7～20nm之间,长度最长可以达到50μm以上.为了结构更加稳定,对分子瓦中每条DNA单链的5′末端进行磷酸化处理,自组装完成后利用T4DNA连接酶连接磷酸化修饰的DNA纳米管的缺口.AFM结果显示,使用T4DNA连接酶处理后的DNA纳米管更能保持完好的管状结构,表明连接处理后的DNA纳米管更加坚固,促进了DNA纳米管应用于微纳米领域的研究.DNA molecular self-assembly technology can be used to build different nanostructures from one dimension to three dimension,and these structures have many potential uses in micro/nano-electronics,nano-biology as well as other fields.We have successfully assembled one dimension DNA nanotubes by using double-crossover（DX） DNA tiles.The nanotube structures were characterized by atomic force microscope（AFM）,native-PAGE gel electrophoresis,fluorescence microscope and transmission electron microscope（TEM）.The results show that the diameter of these DNA nanotubes are between 7 and 20 nm,and the longest diameter can reach to more than 50 μm.To stabilize these nanotubes,each strand was phosphorylated chemically on the 5énd.After assembling the nanotubes,T4 DNA ligase was used to ligate the nicks.AFM detection indicates that the ligated DNA nanotubes can keep intact tubular structure better than the unligated one,which reveals that the ligation method can strengthen the DNA nanotubes.The robust nanostructures would promote the research of DNA nanotubes in applications of micro/nano-technology.

关 键 词：DNA纳米管 DNA分子瓦 黏性末端 磷酸化 DNA连接酶 

分 类 号：TB383.1[一般工业技术—材料科学与工程]