电针对小鼠脑缺血后Neurogenin2表达和神经功能的影响  

Effect of electroacupuncture on Neurogenin2 and neural function after cerebral ischemia in mice

作　　者：张巧梅[1] 李立亚[1] 高淅[1] 王志华[1] 王强[1] 

机构地区：[1]第四军医大学西京医院麻醉科,西安710032

出　　处：《神经解剖学杂志》2013年第3期321-324,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金资助项目(81072888)

摘　　要：目的:探讨电针刺激百会穴对成年小鼠脑缺血损伤后Neurogenin2(Neurog2)的表达和神经功能的影响。方法:成年雄性C57BL/6J小鼠,随机分成4组,对照组,电针+对照组,缺血组,电针+缺血组。结扎双侧颈总动脉构建全脑缺血模型(20 min)。电针组于建模后24 h后连续电针刺激百会穴5 d,采用Real-time PCR方法,检测脑组织中Neurog2 mRNA的表达情况,并且应用total motor scores(TMS)法评估神经功能,分析电针刺激7 d后Neurog2的表达对脑缺血小鼠神经认知的影响。结果:Neurog2基因在脑缺血后3 d表达逐渐增加,5 d最高,7d开始下降,给予电针治疗后,Neurog2的表达较缺血组明显增加,而且电针组的神经行为学评分中也优于缺血组(P<0.05)。结论:脑缺血激活Neurog2基因表达,电针可促进Neurog2表达增加,改善神经功能,这可能是电针治疗脑缺血再灌注损伤的新机制。Objective： To investigate the effect of electroacupuncture（EA） by Baihui acupoint on the expression of Neurogenin2 and neural function in adult mouse after ischemic injury. Methods： Adult male C57BL/6J mice were randomly divided into 4 groups ： sham, sham ＋ EA, ischemia, EA ＋ isehemia. The model was established by bilateral general cervical artery ligation （20 min）. The two EA groups received Baihui electroacupuncture stimulation in the following 5 days. Real-time PCR was used to detect the expression of Neurogenin2 mRNA in the mice brain. Neural function was scored at 7 d after EA treatment to analysis the effect of expression of neurog2 on cognition by using the total motor scores （TMS） method. Results ： The expression of Neurog2 gradually increased at 3 d after cerebral ischemia, reached the peak at 5 d, then it started to decrease at 7 d. After EA treatment, the expression of neurog2 in EA groups showed a significant increase compared with the other two groups. Meantime the neurobehavioral scores was better than the ischemia group （ P 〈 0.05） . Conclusion： Cerebral ischemia can activate the expression of Neurog2, the electroacupuncture can upregulate Neurog2 expression, improve nerve function, it may be a new mechanism that electroacupuncture in the treatment of cerebral ischemia reperfusion injury.

关 键 词：Neurogenin2 电针 神经功能 小鼠 

分 类 号：R245[医药卫生—针灸推拿学]