第三代测序技术及其应用  

The Third Generation Sequencing Technology and Its Application

作　　者：张得芳[1] 马秋月[2] 尹佟明[1] 夏涛[1,2] 

机构地区：[1]1南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京210037 [2]南京林业大学森林资源与环境学院,南京210037

出　　处：《中国生物工程杂志》2013年第5期125-131,共7页China Biotechnology

基　　金：林业公益性行业重大项目(201304102);国家自然基金(31270711)资助项目

摘　　要：第二代测序技术发展到一定阶段以后其缺陷逐渐显现,而第三代测序技术的出现在一定程度上弥补了第二代测序技术在应用方面的缺点。就目前正在发展的5种第三代高通量测序技术的原理进行了阐述,并比较了这几种测序技术的优缺点,最后对三代测序技术在基因组测序,甲基化研究,RNA测序以及医学方面的应用作了简单介绍。With the development and application of next generation sequencing technology （NGS）, some disadvantages are emerging. The third generation single molecular sequencing technology, to a certain extent, may offset these disadvantages of the next generation sequencing in application. This study elaborated the 4 sequencing principles of the third generation single molecular sequencing technologies, compared the advantages and disadvantages with these sequencing technologies, and presented their application in genome sequencing, methylation research, RNA sequencing and medical research.

关 键 词：第三代测序 单分子测序技 术SMRT tSMS 

分 类 号：Q523.8[生物学—生物化学]