叶酸和白藜芦醇对TCDD致胎鼠腭裂的拮抗作用  

The antagonistic effect of folic acid and resveratrol on cleft palate in mice induced by TCDD

作　　者：何晓梦[1] 刘翠苹[1] 甘立强[1] 袁心刚[1] 邱林[1] 田晓菲[1] 刘燕[1] 肖军[1] 魏光辉[1] 傅跃先[1] 

机构地区：[1]重庆医科大学附属儿童医院整形外科、儿童发育疾病教育部重点实验室、重庆市儿科学重点实验室、重庆市国际科技合作基地,重庆400014

出　　处：《中华整形外科杂志》2013年第3期197-201,共5页Chinese Journal of Plastic Surgery

基　　金：重庆市自然科学基金重点项目（CSTC,2009BA5085）

摘　　要：目的探讨叶酸和白藜芦醇是否具有预防动物模型腭裂发生的作用，并对比两者的效果。方法将72只孕鼠随机分为9组，每组8只，2，3，7，8-四氯代二苯并二嗯英（2，3，7，8-tetrachlorodibenzo—P—dioxin，TCDD）组于受孕后第10天（GD10）以TCDD28μg／kg灌胃；叶酸组分别于GD10以15、10、5mg／kg叶酸＋TCDD28μg／kg灌胃，对照组以玉米油0．1ml灌胃；白藜芦醇处理孕鼠分3组，GD8～13组以50mg／kg白藜芦醇于GD8—13灌胃6次，白藜芦醇GD8～13＋TCDD组以白藜芦醇50mg／kg于GD8—13灌胃6次及TCDD28μg／kg于GDl0灌胃1次，白藜芦醇GD10＋TCDD组以白藜芦醇50mg／kg及TCDD28μg／kg于GD10灌胃，对照组以等量羧甲基纤维素钠溶液于GD8～13灌胃及玉米油0．1ml于GD10灌胃。GD17．5处死孕鼠，称量孕鼠及胚胎体重，记录活胎鼠数、腭裂数、吸收胎及死胎鼠数；并剪取胎鼠头部，解剖显微镜下观察。结果TCDD28μg／kg所致胎鼠腭裂发生率为92．86％，对照组未见胎鼠腭裂形成。TCCD＋叶酸15、10、5mg／kg3组致胎鼠腭裂发生率分别为84．00％、73．08％、86．00％。白藜芦醇GD8～13、GD8—13＋TCDD组、GD10＋TCDD3组致胎鼠腭裂发生率分别为0％、57．78％、74．51％。白藜芦醇GD8～13＋TCDD组孕鼠每窝活胎数、死胎和吸收胎数，与对照组及TCDD组相比，差异均有统计学意义（P〈0．05）；其余各实验组孕鼠体重增加量、活胎鼠体重及每窝平均胎数、每窝活胎数与对照组相比，差异均无统计学意义（P〉0．05）。结论试验剂量的叶酸与白藜芦醇均有一定的拮抗TCDD诱导胎鼠腭裂发生的作用，以叶酸10mg／kg、白藜芦醇50mg／kgGD8～13的剂量的拮抗作用较强。两者作用效果无明显差异，但白藜芦醇50mg／kg（GD8～13）明显影响TCDD作用下胎鼠的生长发育。Objective To evaluate whether or not administration of folic acid and resveratrol have preventive effects on c/eft palate formation as well as the comparison of the two drugs＇ s effects. Methods Pregnant mice were randomly divided into 9 groups, with 8 mice in each group. The TCDD group mice were dosed with TCDD 28 μg/kg body weight on gestation day 10 （ GD 10） ; animals in folic acid group were respectively dosed with folic acid 15,10,5 mg/kg and TCDD 28 μg/kg; resveratrol treated mice were divided into 3 groups： resveratrol 50 mg/kg were orally administered for 6 consecutive days, from gestational day GD 8 to GD13 in resveratrol （GD8-13） group; resveratrol 50 mg/kg were orally administered for 6 consecutive days, from gestational day GD 8 to GD13, followed by an oral administeredwith TCDD on GD10 in resveratrol （GD8-13） ＋ TCDD group; resveratrol 50mg/kg and TCDD 28 μg/kg were used by gavage administration at GD10 in resveratrol （GD10） ＋ TCDD group. Control mice were treated with the same volume of water for 6 consecutive days from GDS to GD13 and were given a single dose of corn oil on GD10. The pregnant mice weight and embryos, the number of live, cleft palate, dead and resorption fetal mice were recorded on GD 17.5. The coronal sections of the fetal mice heads were prepared at GD 17.5 and observed by microscopy. Results Total frequency of clefts was 92. 86% in TCDD group,84.00% （ 15 mg） ,73.08% （ 10 mg） ,84. 00% （5 mg） in folic acid ＋ TCDD groups,0% in resveratrol （GD10） group, 74.51% （GD10） , 57.78% （ GD8-13 ） in resveratrol ＋ TCDD groups. The frequency of cleft was 0% in the control group. Compared with the control and the TCDD groups, there were significant differences in the number of live, dead and resorption fetal mice in TCCD ＋ resveratrol （GD8-13）group（ P 〈 0.05 ）. No significant differences in embryonic weight, live fetuses weight, the number of live, dead and resorption fetal mice were found in the other groups （P 〉 0.05 ）. Co

关 键 词：叶酸 白藜芦醇 2 3 7 8-四氯代二苯并二噁英 腭裂 小鼠 

分 类 号：R28[医药卫生—中药学]