落叶松毛虫AChE最佳反应体系建立及其对3种杀虫剂敏感性比较  

Determination of optimized reaction system for AChE in Dendrolimus superans and comparison of its sensitivity to three pesticides.

作　　者：邹传山[1] 曹传旺[1] 王志英[1] 

机构地区：[1]东北林业大学林学院

出　　处：《北京林业大学学报》2013年第3期102-107,共6页Journal of Beijing Forestry University

基　　金：林业公益性行业科研专项(201104069);黑龙江省科技计划项目(GA09B203-3);中央高校基本科研业务费专项资金项目(DL11BA17)

摘　　要：通过正交试验和酶活性测定法研究了落叶松毛虫6龄幼虫乙酰胆碱酯酶(AChE)最佳反应体系及对3种杀虫剂的敏感性。结果表明:落叶松毛虫6龄幼虫AChE最佳反应条件为酶液质量浓度0.2g/mL、底物浓度0.8mmol/L、反应体系pH7.5、水浴温度35℃和反应时间5min。敌敌畏、高效氯氟氰菊酯和阿维菌素对落叶松毛虫6龄幼虫体外AChE活性产生不同的抑制作用,且抑制效应与杀虫剂呈现明显的剂量--效应关系;其中敌敌畏抑制效果最显著(IC50=3.018×10-4mg/mL),其次是高效氯氟氰菊酯(IC50=1.436×10-2mg/mL),而阿维菌素对AChE活性抑制效果最弱。In this study, the optimal reaction system of acetylcholinesterase （ACHE） in 6th instar larval Dendrolimus superans was determined by orthogonal matrix method, then sensitivities of AChE to DDVP, lambda-cyhalothrin and avermectins were compared through enzyme assay. The results showed that the optimal conditions of AChE activity assay in 6th instar larval D. superans were 0.2 g/mL of AChE concentration, 0.8 mmol/L of substrate concentration, pH 7.5, 35 ℃ of temperature and 5 minutes of reaction time. Moreover, the three pesticides, DDVP, lambda-cyhalothrin and avermectins, showed different inhibition to in vitro AChE activity in 6th instar larval D. superans. Relationship of inhibitory effect and pesticides was in a concentration way. Among three pesticides, DDVP showed the most significant inhibition to AChE activity in 6th instar larval D. superans （ ICs0 = 3. 018 ~ 10 -4 mg/mL）, following lambda-cyhalothrin（ICs0 = 1. 436 × 10-2 mg/mL） , whereas avermectin was the weakest. These results will provide basal information for further studying the biochemical characteristics of AChE and toxicity mechanisms of pesticides.

关 键 词：落叶松毛虫 乙酰胆碱酯酶活性 正交试验 杀虫剂 

分 类 号：S763.3[农业科学—森林保护学]