正常木与应压木木材形成组织中差异表达蛋白质的鉴定  

Identification of differential protein in the wood forming tissue of normal wood and reaction wood.

作　　者：石江涛[1,2] 刘一星[2] 聂玉哲[3] 李坚[2] 

机构地区：[1]南京林业大学木材工业学院 [2]东北林业大学材料科学与工程学院 [3]东北林业大学大庆生物技术研究院

出　　处：《北京林业大学学报》2013年第3期137-142,共6页Journal of Beijing Forestry University

基　　金：国家外专局高等学校学科创新引智计划项目(B08016)

摘　　要：为了鉴定应压木木材细胞壁形成中的差异表达蛋白质,并确定其在木材形成中的功能,采用双向差异凝胶电泳技术,对正常木和应压木木材形成组织中蛋白质进行分离和鉴定,获得了较高质量的双向凝胶电泳图谱。应用TOF-MS/MS技术对应压木和正常木木材形成组织中的差异表达蛋白质进行鉴定,并用MASCOT软件对每个差异点的匹配峰值在SwissProt数据库中进行检索,最终确定了7个差异蛋白质。其中,4个蛋白质在应压木中表达上调,3个表达下调。这些蛋白质参与了木质素生物合成、应力响应与胁迫、细胞骨架运动及脂类代谢,因而推测它们与应压木木材的形成有关。In order to identify the differential proteins in compression wood cell wall formation and determine their function in wood formation, two-dimension difference in-gel electrophoresis was employed to separate and identify differential proteins in wood forming tissue of normal and compression wood. The gel electrophoresis map was higher performance. The differential expressed protein was analyzed and found that the changing fold of seventeen spots was more than twice. Mass identification was carried out using TOF-MS/MS and the peptide fragments were searched in SwissProt database by MASCOT software. Seven proteins were confirmed, four were up regulated and three were down regulated in compression wood. These proteins were involved in lignin biosynthesis, stress response, cytoskeleton motion and lipid metabolism. Presumably, they were connected with the formation of compression wood.

关 键 词：应压木 木材形成 差异蛋白质 

分 类 号：S781.1[农业科学—木材科学与技术]