鸟结核分枝杆菌感染巨噬细胞的分子免疫机制研究  

Molecular immune mechanisms of macrophages infected with Mycobacterium avium

作　　者：王建军 汤立军[2] 陈偲[2] 罗小梅[2] 潘艺[2] 谢萍芳[3] 

机构地区：[1]江苏省昆山市第一人民医院,昆山215300 [2]中南大学生命科学学院分子生物学研究中心,长沙410078 [3]中南大学湘雅二医院,长沙410011

出　　处：《中国免疫学杂志》2013年第5期468-473,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(No.81071326);中南大学大学生创新创业训练计划(YC12375)

摘　　要：目的:探讨鸟结核分枝杆菌感染巨噬细胞后产生的免疫防御分子机制。方法:通过抗酸染色分析巨噬细胞受M.avium感染后吞噬M.avium的能力;并运用real-time PCR及流式细胞术从基因和蛋白水平检测巨噬细胞受M.avium感染后细胞CD80、CD86的表达情况;同时以ELISA方法检测M.avium感染巨噬细胞后细胞上清中IFN-γ、TNF-α含量。结果:巨噬细胞随着时间变化吞噬M.avium的量不断增加;real-time PCR与流式细胞术结果表明巨噬细胞在受M.avium感染后CD80、CD86基因与蛋白表达上调。同时ELISA检测结果表明IFN-γ、TNF-α在M.avium感染巨噬细胞后培养上清中含量增加。结论:巨噬细胞吞噬M.avium的能力随时间变化而增强。M.avium可促进巨噬细胞表面信号分子CD80、CD86在基因及蛋白水平表达表达增强,并促进巨噬细胞TNF-α、IFN-γ的分泌从而增强巨噬细胞的炎症反应。Objective：To explore the molecular immune mechanisms of macrophages defense Mycobacterium tuberculosis.Methods： Acid-fast staining was adopted to analyze phagocytosis of macrophages infected with M.avium.CD80,CD86 expression on macrophage were detected by real-time PCR and flow cytometry technology;and IFN-γ,TNF-α in the supernatant secreted from macrophage infected with M.avium were determined by ELISA.Results： The amount of M.avium engulfed by macrophages increased with extended incubation time;CD80,CD86 were upregulated at gene and protein levels after macrophages infected with M.avium by real-time PCR and flow cytometry analysis.Meanwhile IFN-γ,TNF-α in cell culture supernatant were increased after macrophage stimulated with M.avium by ELISA methods.Conclusion： The phagocytosis ability of macrophage changed with time increasing.M.avium enhanced the expression of CD80 and CD86 on macrophage at gene and protein levels,and then stimulated macrophage to secret TNF-α,IFN-γ to accelerate inflammatory response of macrophage.

关 键 词：巨噬细胞 M AVIUM CD80 CD86 IFN-γ TNF-α 

分 类 号：R392.11[医药卫生—免疫学]