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机构地区:[1]青岛大学医学院附属医院风湿免疫科,青岛266003
出 处:《中国免疫学杂志》2013年第5期508-510,共3页Chinese Journal of Immunology
基 金:山东省医药卫生科研项目(No.HW027)
摘 要:目的:探讨系统性红斑狼疮(SLE)外周血单个核细胞(PBMCs)的c-myc mRNA表达在SLE发病中的作用。方法:从33例SLE患者及20例健康对照组外周静脉血中分离PBMCs,提取总RNA作模板,按文献设计合成c-myc引物,以β-actin作内参,应用半定量逆转录-聚合酶链反应(RT-PCR)检测SLE患者及健康对照的PBMCs c-myc mRNA表达水平并进行组间比较,用系统性红斑狼疮疾病活动性指数(SLEDAI)评定每例患者疾病活动性,并对SLE患者PBMCs的c-myc mRNA表达水平与SLEDAI进行相关分析。结果:c-myc及β-actin的RT-PCR扩增产物电泳显示分别为268和163 bp条带。SLE患者PBMCs的c-myc mRNA相对表达量为0.58±0.26,而正常对照的相对表达量为0.07±0.04,两组差别有显著性(t'=11.024,P=0.000)。25例活动期SLE患者的c-myc mRNA相对表达量为0.62±0.25,而8例缓解稳定期SLE患者的c-myc mRNA相对表达量为0.25±0.01,组间差别也有显著性(t'=7.86,P=0.000)。SLE患者PBMCs的c-myc mRNA相对表达量与SLEDAI呈正相关(r=0.865 1,P<0.05)。结论:SLE患者PBMCs的c-myc mRNA表达异常,c-myc mRNA表达水平与SLE疾病活动评分指数呈直线正相关关系。c-myc mRNA表达水平可作为判断SLE疾病活动性的一个指标。Objective:To investigate the role of the peripheral blood mononuclear cells(PBMCs) c-myc mRNA expression in the pathogenesis of systemic lupus erythematosus(SLE).Methods:The PBMCs were isolated from 33 SLE patients and 20 healthy controls.Total RNA was extracted from PBMCs as template.The specific c-myc primers were designed and synthesized according to reference documentation.The PBMC c-myc mRNA expression level was detected by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR).The SLE disease activity was assessed using systemic lupus erythematosus disease activity index(SLEDAI).The correlation between the of c-myc mRNA expression level and SLEDAI was analyzed by linear correlation.Results:The c-myc mRNA relative expression level of SLE patients was 0.58±0.26,significantly higher than that(0.07±0.04) of normal control(t′=11.024,P=0.000);the c-myc mRNA relative expression level of SLE patients with active disease was 0.62±0.25,significantly higher than that(0.25±0.01) of SLE patients with in remission(t′=7.86,P=0.000).There was a positive correlation between the c-myc mRNA relative expression level and SLEDAI(r=0.865 1,P〈0.05).Conclusion:The c-myc mRNA expression level of SLE was abnormal,and relative positively to SLEDAI.The c-myc expression level may be used as a index for evaluating and monitoring the SLE activity.
关 键 词:系统性红斑狼疮 C-MYC MRNA 逆转录-聚合酶链反应
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