猫主要过敏原Fel d1的T细胞抗原表位分析及三维结构模拟  

Prediction of T cell epitopes and there-dimensional structure of cat major allergen Fel d 1

作　　者：许晨霞[1] 何颖[1] 肖琪[1] 邹泽红[1] 袁旭平[1] 关学琛[1] 

机构地区：[1]广州市过敏反应与临床免疫重点实验室/呼吸疾病国家重点实验室变态反应研究室/广州医学院第二附属医院,广州510260

出　　处：《中国免疫学杂志》2013年第5期530-533,共4页Chinese Journal of Immunology

基　　金：国家重大科技专项重大课题(2011ZX08011-005);广州医学院项目(2006GD053)资助

摘　　要：目的:预测猫主要过敏原Fel d 1与MHCⅠ、MHCⅡ类分子的结合力获得T细胞优势抗原表位,并模拟Fel d 1的三维结构,为猫主要过敏原的改造及其临床研究等提供依据。方法:从Uniprot数据库中得到猫主要过敏原Fel d 1的氨基酸序列,通过在线软件NetMHCⅡ2.2对Fel d 1氨基酸序列进行MHCⅡ抗原表位分析,使用软件SYFPEITHI分析MHCⅠ的抗原表位,再通过在线软件Swiss-Model预测Fel d 1的三维结构,以Ramachandran图评估三维结构的稳定性。结果:运用生物学软件分析得到Fel d 1上两个高值MHCⅡ抗原表位区域分别为22～43、80～95,MHCⅠ抗原表位优势区为17～30、56～84、91～103。Ramachandran图显示Fel d 1的空间构象稳定。结论:本研究有助于确定Fel d 1的T细胞优势抗原表位及其三维结构模型,为Fel d 1抗原性改造提供理论依据,及将来的临床研究奠定基础。Objective：To obtain the T cell dominant epitopes of cat major allergen Fel d 1 by predicting its binding affinity with MHC class Ⅰ and Ⅱ,and to model the three-dimensional structure of Fel d 1,so as to provide a novel clue for modifying the antigenicity and clinical research of Fel d 1.Methods： The protein sequence of Fel d 1 was extracted from the UniProtKB.We predicted MHCⅡ epitopes of Fel d 1 by online software NetMHCⅡ 2.2 Server and predicted its MHCⅠ epitopes by SYFPEITHI.The three-dimensional structure modeling of Fel d 1 was done using SWISSMODEL.The stability of its spatial conformation was evaluated by Ramachandran plot.Results： The predicted results showed that the dominant regions of MHCⅡ epitopes on Fel d 1 were 22-43 and 80-95,and the dominant regions of MHCⅠ epitopes were 17-30,56-84 and 91-103.The Ramachandran plot showed the spatial conformation of Fel d 1 was stable.Conclusion： The study helps to determine the T cell dominant epitopes and the three dimensional structure of Fel d 1,which provide a theoretical basis for antigenic modification and lay the foundation for future clinical study.

关 键 词：猫主要过敏原 FEL D1 抗原表位 三维结构 

分 类 号：R392.8[医药卫生—免疫学]