山东地区新城疫病毒分离株的分离鉴定及其F基因的分子特性分析  

Isolation,Identification and Molecular Characterization for FGene of Newcastle Disease Virus Isolated in Shandong

作　　者：杨金兴[1] 朱瑞良[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《华北农学报》2013年第2期231-234,共4页Acta Agriculturae Boreali-Sinica

基　　金：山东省自然科学基金项目(ZR2010CQ044)

摘　　要：2011年从山东不同地区的发病鸡体内分离到4株新城疫病毒,分别命名为SD1～SD4。4株毒株经病毒蚀斑克隆纯化后动物回归试验可产生典型的NDV病变;为了解其遗传起源和分子特性对其融合蛋白(Fusion,F)进行序列和分子特性的分析。研究结果发现,生物学毒力测定显示4株毒株均为强毒。F蛋白多肽裂解位点的序列为112RRQKRF117,符合NDV强毒氨基酸基序。F基因分型显示,SD01、SD02、SD03和SD04与另外一株鸡源SG01以及广东鹅分离株等属于基因Ⅶ型。同源性比较显示:SD1-SD4与同期分离株的同源性明显高于其他的传统毒株,且与国内分离株的同源性高于国外的分离株。Four Newcastle Disease Virus isolates named SD01-SD04 were collected from chicken in Shandong in 2011.The four strains were purified by plaque assay can form typical cytopathic.The F genes of the isolates were cloned and sequenced to study its molecular characterization and hereditary variation.The deduced amino acid sequence of F gene showed that these viruses shared a velogenic motif 112 RRQKRF 117 at the cleavage site which were coincident with biological index.Phylogenetic analysis showed that SD01-SD04 and the viruses derived from chicken and goose in China belonged to genotype Ⅶ.For F gene of the isolates,the homologies of the deduced amino acid sequences of SD01-SD04 with the same time isolates in China were higher compared with traditional trains.There was a closer genetic relationship between the four isolates and epidemic strains in China than foreign epidemic strains.

关 键 词：新城疫病毒 F基因 分子特性 

分 类 号：S432.4[农业科学—植物病理学]