红毛五加多糖不同组分对小鼠腹腔巨噬细胞免疫功能的研究  

Effect of Different Polysaccharide Fraction from Acanthopanax Giraldii Harms on Peritoneal Macrophage

作　　者：郝慧慧[1] 陈永[2] 林志娟[2] 张义军[1] 

机构地区：[1]潍坊医学院药学与生物科学学院 [2]潍坊医学院基础医学院.潍坊261002

出　　处：《天然产物研究与开发》2013年第5期612-616,共5页Natural Product Research and Development

基　　金：山东省自然科学基金项目(ZR2011HQ044);潍坊市科学技术发展计划项目(201101178)

摘　　要：本文研究红毛五加多糖不同组分(AHP-I、AHP-II、AHP-III)对小鼠腹腔巨噬细胞免疫调节功能的影响,为进一步阐明红毛五加多糖对小鼠免疫调节作用机制奠定基础。采用不同浓度的3种多糖组分作用于小鼠腹腔巨噬细胞,测定其对巨噬细胞吞噬中性红、释放NO能力、分泌IL-6、TNF-α、IL-1β水平的影响。最后结果是红毛五加多糖的3种不同组分对小鼠免疫细胞有不同的刺激能力。其中,AHP-II可极其显著地增强吞噬细胞的吞噬功能,促进其合成NO,促进巨噬细胞细胞因子的分泌。因此红毛五加多糖能激活小鼠腹腔巨噬细胞,其中,AHP-II是最重要的作用组分。The objective of study was to investigate the effects of different components of Acanthopanax Giraldii Harms polysaccarides（AHP-I,AHP-II,AHP-III）on the immunoregulation function of the peritoneal macrophage of mouse.The engulfment of neutral red and the expression levels of NO,IL-6,TNF-α,IL-1β were determined for all of these three fractions.The Results showed that the polysaccharides from Acanthopanax Giraldii Harm have different stimulating activities on mouse＇s peritoneal macrophage cells.The AHP-II enhanced the phagocytic activity significantly,and promoted the release of NO and other cytokines.All of the three polysaccharides activated the mouse＇s peritoneal macrophage cells,and the effect of AHP-II was the highest.

关 键 词：红毛五加多糖 腹腔巨噬细胞 免疫调节 NO 细胞因子 

分 类 号：R96[医药卫生—药理学] R392.5[医药卫生—药学]