木犀草素-锡(Ⅱ)配合物的合成、表征及抗氧化活性研究  

Synthesis,Characterization and Antioxidant Activity of Stannum(Ⅱ)-Luteolin Complex

作　　者：夏小明[1,2] 张杰[2] 刘绣华[1,2] 

机构地区：[1]河南大学化学化工学院环境与分析科学研究所 [2]河南大学化学生物学研究所,河南省天然药物与免疫工程重点实验室,开封475004

出　　处：《天然产物研究与开发》2013年第5期656-661,共6页Natural Product Research and Development

基　　金：国家科技支撑计划项目(2011BAI06B04);河南省省院合作项目(102106000021);河南省教育厅资助项目(12B350001)

摘　　要：木犀草素与金属离子有较强的配位能力,形成配合物后,活性会发生变化,因此研究了木犀草素与锡(Ⅱ)配合物的合成及其抗氧化活性。采用紫外可见分光光度法、红外光谱法和核磁共振氢谱法对配合物的结构进行表征,并通过DPPH自由基法和邻二氮菲-Fe2+法分别测定了木犀草素-锡(Ⅱ)配合物对DPPH自由基和羟基自由基(.OH)的清除作用。结果表明,木犀草素与锡(Ⅱ)发生配位的位点在5-OH～4-C=O位和3',4'-OH位,木犀草素-锡(Ⅱ)配合物具备一定的清除DPPH自由基和.OH自由基的性能,但是由于二价锡离子与木犀草素分子中的活性位点(酚羟基)发生了配位络合,所以清除上述自由基的性能较木犀草素均有所降低。Luteolin has powerful complexing properties with metal ion.In this study,the synthesis and antioxidant activity of stannum（II）-luteolin complex were studied.The structure of the complex had been characterized by UV-visible,IR and 1H NMR spectroscopic techniques.The antioxidant activity of the complex was evaluated using 1,1-diphenyl-2-picrylhydrazyl（DPPH）and 1,10-phenanthroline-Fe2＋（OH）methods.The results showed that the coordination sites of luteolin with stannum（II）cations were 5-hydroxy-4-carbonyl and 3′,4′-dihydroxyl（catechol）.Luteolin-Sn（II）complex showed weaker antioxidant activities than luteolin,which was due to the complexation of Sn（II）with the active site at 5-OH^4-C=O and 3′,4′-OH in the molecule of luteolin.

关 键 词：木犀草素 锡(Ⅱ)配合物 自由基 抗氧化 

分 类 号：O657.3[理学—分析化学] R285.5[理学—化学]