大鼠UQCRFS1基因的克隆及其表达模式分析  

Cloning and Expression Pattern of UQCRFS1 Gene in Rattus norvegicus

作　　者：张婷[1] 黄青松[2] 范雪晖[3] 

机构地区：[1]新乡医学院三全学院,河南新乡453000 [2]新乡医学院微生物教研室,河南新乡453000 [3]新乡医学院生命科学技术学院,河南新乡453000

出　　处：《湖北农业科学》2013年第9期2174-2177,共4页Hubei Agricultural Sciences

基　　金：新乡医学院高学历人才资助项目

摘　　要：通过克隆获得泛醌-细胞色素C还原酶铁硫蛋白亚基1(Ubiquinol-cytochrome C reductase iron-sulfur subunit 1,UQCRFS1)基因,对基因序列及其编码的蛋白质进行分析,研究了其在大鼠(Rattusnorvegicus)心、脑、肾、肺组织中的表达模式。测序结果显示UQCRFS1基因全长826 bp,RT-PCR结果显示其在大鼠心脏中表达量较高,脑和肺中表达量稍低,肾脏组织中表达量最低,推测UQCRFS1的功能可能与大鼠的心脏发育有关。The sequence of UQCRFS1 （Ubiquinol-cytochrome C reductase iron-sulfur subunit 1） gene was obtained by cloning; the gene sequence and encoded protein were analyzed. Its expression pattern in heart, brain, kidney, and lung tis- sues of rat（Rattus norvegicus） was detected by RT-PCR. The overall length of the UQCRFS1 gene was 862bp, RT-PCR re- suits showed that its expression was the highest in heart tissue of rat, lower in brain aud lung tissue, and the lowest in kid- ney tissue, suggesting that UQCRFS1 was related with the development of heart in rat.

关 键 词：大鼠(Rattus norvegicus) UQCRFS1基因 克隆 表达模式 

分 类 号：Q786[生物学—分子生物学]