国产二甲基二丁基氯化胺试剂替代凝聚胺检测IgG类血型抗体的初步应用  

Establishment and initial application of PDC reagent for detection of IgG blood group antibodies

作　　者：周同彬[1] 栾建凤[1] 雷千红[1] 叶东[1] 赵飞浪[1] 谭明乾[2] 陆路德[2] 杨绪杰[2] 

机构地区：[1]南京军区南京总医院输血科,南京210002 [2]南京理工大学化工学院

出　　处：《临床检验杂志》2000年第4期205-206,共2页Chinese Journal of Clinical Laboratory Science

摘　　要：利用国内自行合成的聚胺阳离子 (Polycation)材料二甲基二丁基氯化胺及数种廉价辅助材料成功地建立了代替进口凝聚胺 (polybrene)技术检测IgG类血型抗体的PDC三试剂法。本法不仅比传统方法操作简便、作用时间短、灵敏度高 ,且达到了进口凝聚胺的技术水平。本法原理同凝聚胺技术 ,可用于血型鉴定 ,交叉配血、抗体筛选。另外 ,对弱的IgM类血型抗体的反应也有明显的增强作用。PDC method was established with national synthetic polycation (Polymer dimethyldibutylammonium chloride,PDC and other cheap materals to replace import polybrene for detection of IgG blood group antibodies.Compared with tranditional methods,this method are simplicity,rapidity,high sensitivity,also as polybrene Its principle is as polybrenes,it can be used for blood grouping,cross matching and antibodies screening test.In addition,it can strengthen reaction of weaker IgM blood group antibodies.

关 键 词：IgG类血型抗体 二甲基二丁基氯化胺试剂 PDC 

分 类 号：R446.62[医药卫生—诊断学]