应用表型系统聚类和SSR分子标记研究水稻亲本及籼粳衍生系遗传多样性  

Genetic Diversity of Parents and Derived Lines Based on Phenotypic Characters and SSR Cluster Analysis

作　　者：杨旺兴 许旭明 祁建民[2] 卓伟 张受刚 马彬林 韦新宇 邹文广 

机构地区：[1]福建省三明市农业科学研究院/国家水稻产业体系三明综合试验站,福建沙县365509 [2]福建农林大学生命科学院,福建福州350002

出　　处：《福建农业学报》2013年第3期211-216,共6页Fujian Journal of Agricultural Sciences

基　　金：国家科技支撑计划子课题(2006BAD01A01-3);福建省科技计划项目(2007N0070)

摘　　要：以17份籼稻、粳稻亲本及39份籼粳亚种间杂交衍生系为材料,利用SSR分子标记和表型系统聚类进行类群划分和遗传多样性分析。类群结果表明:分子标记聚类与表型聚类结果较吻合,均能较准确地将优势类群聚在一起。表型聚类不能准确反映供试材料之间的亲缘关系,而分子标记聚类可以从分子水平上反映各品种间的差异,并能较准确地反映供试材料间的亲缘关系,说明SSR分子聚类适用于亲缘关系不明晰材料的亲缘分析及优势类群划分。以221对SSR引物对试验材料进行遗传多样性分析,结果表明籼粳交衍生系的多样性Nei指数、多样性Shannon指数、PIC多态指数最大值均大于亲本,Nei基因多样性指数最大值为0.819,亲本为0.796;Shannon多样性指数最大值为1.856,亲本为1.677;多态信息含量(PIC)最大值为0.796,亲本为0.766;表明已经选育出的部分籼粳亚种间杂交衍生系有着比供试亲本更丰富的等位基因变异。Using 39 Minghui derived lines from indica-japonica cross and 17 indica and japonica parents as materials,the heterotic grouping and the genetic diversity analysis were researched.The result of the cluster analysis with SSR molecular markers was similar to it of the cluster analysis with phenotype on accurately clustering dominant groups.However,the cluster analysis with phenotypic traits did not accurately indicate the relationship between tested materials but SSR markers did it at the molecular level.It suggests that the cluster analysis of SSR molecular markers could be applied to unknown materials in relationship for the heterotic grouping.SSR markers of 221 primer pairs were used to analyze genetic diversity of tested materials.The results showed that,the maximum values of Nei gene diversity index,Shannon diversity index and polymorphism information content（PIC） were all higher in the strains of indica-japonica cross than in parental values.The maximum value of Nei gene diversity index,Shannon diversity index,polymorphism information content（PIC） were 0.819,1.856,0.796 for the strains of indica-japonica cross,respectively,and 0.796,1.677,0.766 for their parents′respectively.It indicated that,by genetic selection,the allelic variation was more abundant in the strains of indica-japonica cross than in the parental.

关 键 词：水稻 籼粳杂交 衍生系 微卫星标记 遗传多样性 

分 类 号：S511[农业科学—作物学]