草莓离体再生和种苗繁育体系的建立  

Establishment of Regeneration and Propagation System of Strawberry

作　　者：朱海生[1,2,3] 花秀凤[4] 林珲[1,2,3] 陈敏氡[5] 温庆放[1,2,3] 

机构地区：[1]福建省农业科学院作物研究所,福建福州350013 [2]福建省农业科学院蔬菜研究中心,福建福州350013 [3]福建省蔬菜工程技术研究中心,福建福州350013 [4]福州市蔬菜科学研究所,福建福州350012 [5]福建农林大学园艺学院,福建福州350002

出　　处：《福建农业学报》2013年第3期227-231,共5页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31101534);福建省自然科学基金项目(2010J0111);福建省财政专项--福建省农业科学院科技创新团队建设项目(CXTD2011-20)

摘　　要：以‘福莓一号’和‘红实美’草莓为材料,建立了草莓叶片高效离体再生系统,以茎尖为外植体,建立了茎尖脱毒体系。结果表明,‘福莓一号’叶片最佳芽诱导培养基为MS+2.0mg.L-1 6-BA+0.1mg.L-12,4-D,诱导率可达85.7%,‘红实美’叶片最佳芽诱导培养基为MS+2.0mg.L-1 6-BA+0.1mg.L-1IBA,诱导率可达81.9%;2个品种茎尖最佳诱导和增殖培养基为MS+0.5mg.L-1 6-BA+0.1mg.L-1NAA,芽增殖系数为8.78,同时建立一个草莓茎尖培养、组培苗快繁及种苗繁育体系。We established a rapid propagation and leave regeneration system of strawberry with the stem tip culture of the Hongshimei and Fumei1 strawberry.Our results showed that the medium MS＋2.0 mg·L-1 6-BA ＋ 0.1 mg·L-12,4-D has the optimum induction of leaves shoot for Fumei1 and reaches 85.7%.In the contrast,the medium of MS＋2.0 mg·L-1 6-BA ＋ 0.1 mg·L-1 IBA had the optimum induction of leaves shoot for Hongshimei and reached 81.9%.Meanwhile,the medium of MS＋0.5 mg·L-1 6-BA ＋ 0.1 mg·L-1 NAA had better induction and propagation of stem tip and the propagation coefficient reached 8.78.In the end,an effective system of stem tip cultivation,multiplication and propagation technology of the virus-free seedling of strawberry was established.

关 键 词：草莓 叶片再生 茎尖培养 脱毒苗 

分 类 号：S66[农业科学—果树学]