水稻叶片灰白转黄突变体pyr1的鉴定与基因定位  

Identification and Gene Mapping of Leaf Pale Yellow-Revertible Mutant pyr1 in Rice

作　　者：程欣[1] 任德勇[1] 马娇[1] 朱晓燕[1] 桑贤春[1] 凌英华[1] 赵芳明[1] 何光华[1] 

机构地区：[1]西南大学水稻研究所/转基因植物与安全控制重庆市市级重点实验室/南方山地农业教育部工程研究中心,重庆400716

出　　处：《作物学报》2013年第6期992-998,共7页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2011AA10A100);国家自然科学基金项目(31171178);重庆市攻关项目(CSTC;2012ggc8002)资助

摘　　要：鉴定和克隆叶色突变基因对于深入了解叶绿素合成、降解途径的关系以及植物的光合作用有着重要的作用。从EMS诱变恢复系缙恢10号后代中鉴定出1个灰白转黄突变体pyr1,该突变体在苗期部分死亡,整张叶片呈现灰白色,在不同的生育时期叶片呈现不同的颜色,直到孕穗期叶片上部和叶缘表现黄色。苗期到抽穗期突变体叶绿素含量比野生型显著或极显著降低。透射电镜观察表明,突变体与野生型细胞结构无明显差异,但叶绿体发育异常,内部大量降解,基质片层退化。遗传分析表明该性状受1对隐性基因控制,利用326株F2隐性定位群体将PYR1基因定位在第1染色体长臂上,位于标记RM11722和Ind1之间,物理距离约92kb,本研究为PYR1基因的图位克隆奠定了基础。It is important for the further understanding of the relationship between chlorophyll synthesis and degradation pathways and plant photosynthesis to identify and clone leaf color mutant gene. A leaf pale yellow-revertible mutant temporarily designated as pyrl was obtainted from the progeny office （Oryza sativa L.） restorer line Jinhui 10 which was induced by ethyl methane sul- fonate （EMS）. In the seedling stage, the whole leaf of mutants presented pale and some mutants died. Thepyrl displayed different colors in different growth periods. At the booting stage upper-leaf and leaf margin exhibited yellow. Compared with the wild type, the chlorophyl contents ofpyrl mutant decreased from seedling stage to filling stage. Transmission electronic microscopy obser- vation showed that the structure of cells had no obvious differences between mutant and wild type, but the chloroplast developed abnormally with degradation of the inside and matrix slices. Genetic analysis revealed that the trait was controlled by one reces- sive gene. With 326 recessive individuals from the F2 segregation population, the PYR1 gene was finally mapped between RMl1722 and Indl on the long arm of chromosome 1, with an approximate physical distance of 92 kb. These results provide a basis ofPYR1 gene cloning by map-based strategy.

关 键 词：灰白叶 水稻(Oryza SATIVA L ) 叶绿体 基因定位 

分 类 号：S511[农业科学—作物学]