尖镰孢菌(Fusarium oxysporum)的快速分子检测  

Rapid molecular detection of Fusarium oxysporum by PCR

作　　者：王健生[1] 王佳妹[1] 李潇[1] 董莎萌[1] 张正光[1] 郑小波[1] 

机构地区：[1]南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室,南京210095

出　　处：《植物病理学报》2013年第3期318-322,共5页Acta Phytopathologica Sinica

基　　金：公益性行业(农业)科研专项(200903049-06)

摘　　要：由尖镰孢菌（FusariumoxysporumSchlecht．）引起的大豆枯萎病是危害大豆生产的主要土传病害。该菌在土壤和病残体上均可长期生存造成危害。快速准确地在发病初期植株和带病土壤中进行鉴定和检测对防治该病害至关重要。Based on differences in sterol 14cx-demethylase （CYP51C） sequences of Fusarium genus, two primer pairs, C1/C2 and C3/C4 were synthesized respectively to amplify DNA from Fusarium oxysporum by PCR. More than 45 species of Fusarium and other species of pathogens were used to test the specificity of the primers; C1/C2 amplified only an unique 699 bp band from F. oxysporum. The detection sensitivity with C1/ C2 was 100 ng for genomic DNA of F. oxysporum and 100-microconidia/g soil for the soil pathogens. In con- trast, the nested PCR with two pairs of primers （ C3/C4 and C1/C2 ） increased detection sensitivity to 1 pg for genomic DNA of F. oxysporum and 1-microconidia/g soil for the soil pathogens. And F. oxysporum could be specifically detected by PCR assay with C1/C2 from diseased plant samples.

关 键 词：尖镰孢菌 分子检测 土传病害 大豆生产 枯萎病 病残体 危害 土壤 

分 类 号：S651[农业科学—果树学]