马钱科钩吻生物碱Sempervirine的分离及其抗烟草花叶病毒的活性  

Isolation of Sempervirine with Its Anti-TMV Activity

作　　者：张振[1] 闫晓慧[2] 穆淑珍[3] 郝小江[3] 

机构地区：[1]贵州大学化学与化工学院,贵州贵阳550025 [2]西南林业大学林学院,云南昆明650224 [3]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002

出　　处：《贵州农业科学》2013年第5期67-69,共3页Guizhou Agricultural Sciences

基　　金：国家自然科学基金(31200265;31160374);贵州大学博士基金(X101027)

摘　　要：为了解从马钱科钩吻(G.elegans)中分离出Yohimbane-type生物碱Sempervirine对烟草花叶病毒的抑制作用,以烟草花叶病毒(TMV)为供试病毒,进行了TMV的抑制试验。结果表明:化合物浓度为50μg/mL时,采用活体半叶枯斑法筛选Sempervirine对TMV的侵染抑制率达52.6%;采用离体半叶枯斑法筛选对病毒增殖的抑制活性率为73.3%。试验结果可为今后Sempervirine抗TMV病毒抑制剂的研究开发提供参考。Sempervirine, a yohimbane-type alkaloid, was isolated and identificated from G. elegans, the antiviral activity on tobacco masaic virus （TMV） was determined through an half-leaf necrosis method. The results showed that at 50 ug/mL the obvious activity on TMV infection and proliferation with the inhibition rate reached 52.6% in vivo and 73.3% in vitro relatively. The study provided the elementary data for the further research.

关 键 词：钩吻 Sempervirine 烟草花叶病毒 半叶枯斑法 生物碱 

分 类 号：S43[农业科学—农业昆虫与害虫防治]