MMP-9、BAFF在原发性干燥综合征患者唇腺组织中表达的相关研究  

Expression of MMP-9 and BAFF in the Labial Gland of Patients with Primary Sjgren's Syndrome

作　　者：侯西倩[1] 竺红[2] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院风湿科,银川750004

出　　处：《宁夏医科大学学报》2013年第4期424-427,F0004,共5页Journal of Ningxia Medical University

基　　金：宁夏医科大学重点孵育项目(2010年)

摘　　要：目的探讨基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)、B细胞激活因子(B cell activatingfactor of the tumor necrosis factor family,BAFF)在原发性干燥综合征(PSS)发病过程中的作用和意义。方法采用免疫组化SP法检测50例PSS患者及30例正常对照组唇腺组织中MMP-9、BAFF的表达情况。结果50例PSS患者唇腺组织中MMP-9均阳性高表达,其表达的面积(14382.38±657.39)μm2、累积光密度(2705.46±868.76)均高于正常对照组(8445.10±361.84)μm2、(1114.26±570.82),且随着淋巴细胞浸润等级的增加,MMP-9表达增强,差异有统计学意义(P<0.05);50例PSS组患者唇腺组织中BAFF均阳性表达,其表达的面积(13586.72±1569.45)μm2、累积光密度(2073.57±850.73)均高于正常对照组(7116.83±338.19)μm2、(850.73±120.31),且随着淋巴细胞浸润等级的增加,BAFF表达增强(P<0.05);PSS组患者唇腺组织中MMP-9与BAFF的表达呈正相关(P<0.05)。结论 MMP-9、BAFF的异常表达与原发性干燥综合征患者唇腺组织的破坏有关,二者参与了PSS的发病过程。Objective To investigate the expression of matrix metalloproteinase - 9 （ MMP - 9） and B cell ac-tivating factor of the tumor necrosis factor family （BAFF） in the labial gland of patients with Primary Sjōgren＇s Syndrome （PSS） and to analyse their roles in the pathogenesis of PSS. Methods The expression of MMP - 9and BAFF were detected by the Immunohistochemical method. The labial gland were obtained from 50 pa-tients with PSS and 30 healthy controls. Results The MMP-9 protein and BAFF protein were both over - expressed in the labial gland of patients with PSS. The mean over - expression level （ 14382.38 ± 657.39 ） μm^2, integrated optical density （2705.46 ± 868.76 ）of MMP-9 and the mean over -expression level （ 13586. 72 ± 1569.45 ）μm^2, integrated optical density （2073.57 ± 850.73 ） of BAFF in PPS patients＇ tissues were all significantly higher than those in normal salivary tissues （ 8445. 10 ± 361.84, 1114.26 ± 570.82 ; 7116.83 ±338.19, 850.73 ± 120.31 ） ,P 〈0.05. There was a positive correlation between the expression of MMP - 9and BAFF （ P 〈 0.05 ）. Conclusion The over -expression of MMP - 9and BAFF and the interaction between them in the labial gland of patients with PSS may play an important role in the pathogeny of PSS.

关 键 词：干燥综合征 唇腺疾病 免疫组织化学 基质金属蛋白酶-9 B细胞激活因子 

分 类 号：R593.2[医药卫生—内科学]