新疆哈萨克绵羊MHCⅠ类基因第二、三外显子序列多态性分析  

Sequential Polymorphism Analysis of MHC Ⅰ Gene Exon2 and 3 in Kazakh Sheep

作　　者：刘志方[1] 吴长新[1] 张辉[1] 李蕊[1] 郭吉星[1] 肖红冉[1] 陈创夫[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2013年第2期170-175,共6页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金资助项目(31172303)

摘　　要：为研究新疆中国哈萨克绵羊主要组织性抗原复合体Ⅰ类基因第二、三外显子的序列多态性,采用PCR和测序的方法对新疆伊犁地区36只哈萨克绵羊MHCⅠ类基因第二、三外显子进行了扩增和测序,并对序列进行了生物信息学分析。结果表明:哈萨克绵羊MHCⅠ类基因第二、三外显子序列具有丰富的多态性,碱基组成具有一定的不平衡性,并且经过了自然选择的作用,与数据库中牛的序列的进化分析显示了跨物种多态性。实验数据为深入研究绵羊MHCⅠ类基因的多态性及绵羊抗病新品种的选育提供理论依据。To investigate the genetic polymorphism of the exon2 and 3 of MHC Ⅰ loci in Kazakh sheep. We amplified and se-quenced the exon 2 and 3 of MHC Ⅰ gene in 36 Kazakh sheep from Yili,Xinjiang,and analyzed these sequences with bioinforma-tics software. Results show that the sequences of the exon2 and 3 of MHC Ⅰgene display highly nucleotide diversity and unbal-anced base composition. This gene is the result of natural selection, and shows cross-species polymorphism when compared with the bovine sequences in NCBI Database. The date may be of great help in the further study of the ovine MHC gene and provide basis for the development of the selected breeding of disease resistance.

关 键 词：MHC 聚合酶链式反应 基因多态性 哈萨克绵羊 

分 类 号：S826[农业科学—畜牧学] Q75[农业科学—畜牧兽医]