大豆异黄酮对大鼠肠道上皮内淋巴细胞、杯状细胞及瘦素长型受体的影响  

Effect of soy isoflavones on intraepithelial lymphocytes,goblet cells and long leptin receptor in intestinal of rats

作　　者：唐秀莹[1] 陈正礼[1,2,3] 罗启慧[2,3] 张小龙[1] 

机构地区：[1]四川农业大学动物医学院,动物疾病与人类健康中心四川省重点实验室,四川雅安625014 [2]四川农业大学,实验动物工程技术中心/国家实验用猕猴种源基地,四川雅安625014 [3]四川农业大学预防兽医研究所,成都611130

出　　处：《浙江大学学报（农业与生命科学版）》2013年第3期343-350,共8页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：教育部“长江学者和创新团队发展计划”创新团队资助项目(IRT0848);四川省科技厅应用基础研究资助项目(2011JY0054)

摘　　要：大豆异黄酮具有广泛的生理作用,其对免疫系统的影响近年来成为一个焦点。为了研究大豆异黄酮对肠黏膜屏障结构的重要组成部分(上皮内淋巴细胞、杯状细胞)及免疫调节因子瘦素受体(长型)的影响,本实验采用高脂饲料喂饲大鼠,建立肥胖模型;然后将筛选出的肥胖大鼠分别灌胃剂量为对照(I:0mg/kg)、低(Ⅱ:50mg/kg)、中(Ⅲ:150mg/kg)、高剂量(Ⅳ:450mg/kg)的大豆异黄酮,并设置基础对照组(Ⅴ:溶媒为0.5%羧甲基纤维素钠),连续4周用HE、PAS染色观察小肠上皮内淋巴细胞、杯状细胞数量和分布变化;用免疫组织化学SABC法测定瘦素受体(长型)水平。结果表明:大鼠上皮内淋巴细胞以小型淋巴细胞为主,主要分布于上皮的基底膜附近;大鼠杯状细胞分布在肠黏膜上皮层;瘦素受体(长型)阳性细胞分布于黏膜层。应用大豆异黄酮高剂量组较对照组大鼠的肠黏膜上皮内淋巴细胞数量和杯状细胞的数量明显增加,且有向肠内层移动趋势,同时肠黏膜瘦素受体(长型)水平升高,差异有统计学意义(P<0.05)。表明大豆异黄酮在一定程度上可促进大鼠小肠黏膜屏障结构趋于完整并提高其免疫功能。Summary Soy isoflavones （SIF） are a research focus due to their potential health effects. Some studies have shown that it could modulate immune function and prevention of chronic diseases, hut until now it remains controversial. Thus, the aim of the present study is to investigate the effect of high-fat diet induced obesity on male immune parameters. Numerous studies have reported that immunomodulatory activity of intestinal mucosal barrier was related to intraepithelial lymphocytes （IEL） and goblet cells （GC）. Leptin is secreted almost exclusively by adipocytes. Previous researches demonstrated that plasma leptin levels in ovariectomized rats was lower than that in estradiol-treated animals. Moreover, there was a tissue-specific regulation of the leptin receptor （OB Rb）, with less sensitivity to leptin in adipose tissue when the time of treatment or the dose of estradiol administered increased. The metabolites of soy isoflavones showed structural and functional similarities to estradiol. Available data indicated that soy isoflavones inhibited the leptin secretion, and thus influenced the expression of OB-Rb. The study was divided into two steps. In the first experiment, male Sprague-Dawley rats were fed with a high sugar and fat diet （HSF） or basal diet for eight weeks. Food intake and animal mass were recorded weekly. In the second experiment, 40 selected obese rats were randomly divided into four groups with 10 rats in each group and were fed with soy isoflavones （group I 0 mg/kg, group Ⅱ 50 mg/kg, group Ⅲ 150 mg/kg and group IV 450 mg/ kg） and 10 control rats （group V） were fed with basal diet for four weeks. Soy isoflavones was gavaged with 0.5% sodium carboxymethyl cellulose solutions respectively. At the end of the second experiment, rats were slightly anesthetized with sodium pentobarbital ip （40 mg/kg）, and intestinal was taken and fixed in 10% neutral buffered formalin, processed and trimmed, embedded in paraffin. IEL and GC in the villus epithelium were evaluated in

关 键 词：大豆异黄酮 大鼠 上皮内淋巴细胞 杯状细胞 瘦素受体(长型) 

分 类 号：S852.31[农业科学—基础兽医学]