板蓝根二酮B体外抗癌活性研究  

Studies on in vitro Anticancer Activity of Tryptanthrin B

作　　者：梁永红[1] 侯华新[1] 黎丹戎[2] 秦箐[1] 邱莉[1] 吴华慧[1] 

机构地区：[1]广西医科大学,南宁530021 [2]广西肿瘤防治研究所

出　　处：《中草药》2000年第7期531-533,共3页Chinese Traditional and Herbal Drugs

基　　金：广西教育科研资助!项目 ;桂 1999[383] -5

摘　　要：目的 :板蓝根二酮 B抗人肝癌 BEL- 740 2细胞、卵巢癌 A2 780细胞体外活性研究。方法 :MTT法测定板蓝根二酮 B对肝癌 BEL - 740 2细胞和卵巢癌 A2 780细胞的抑制作用 ,集落形成实验观察药物的诱导分化作用 ,PCR- EL ISA试剂盒测定细胞的端粒酶活性。结果 :板蓝根二酮 B具有抑制肝癌 BEL - 740 2细胞、卵巢癌 A2 780细胞增殖的能力 ,其 IC5 0 分别为 8.2 μg/ m L 和 7.8μg/ m L;且具有诱导分化作用 ,可降低端粒酶活性的表达。结论 :研究结果提示板蓝根二酮 B具有体外抗肿瘤活性。To study the in vitro anticancer activity of tryptanthrin B, one of the active constituent of the root of Isatis tinctoria L on BEL-7402 human hepatocellular carcinoma cell and A2780 human ovarian cancer cell Cytotoxicity assay was tested by MTT method Signs of induction differentiation was observed by colony forming units assay Telomerase activity in cell was determined by PCR-ELISA method IC 50 of tryptanthrin B on BEL-7402 and A2780 cell lines were 8 2 μg/mL and 7 8 μg/mL respectively Telomerase activity was dramatically declined and defferentiation of both cancer cell lines was observed Tryptanthrin B possesed antitumor activity in vitro

关 键 词：板蓝根二酮B 抗肿瘤活性 肝癌 卵巢癌 

分 类 号：R285.5[医药卫生—中药学] R286.91[医药卫生—中医学]