甲基叔丁基醚对细胞周期及细胞凋亡的影响  

Effects of methyl tertiary butyl ether on cell cycle and cell apoptosis

作　　者：周伟[1] 黄关麟 张恒 叶舜华[1] 

机构地区：[1]上海医科大学环境卫生学教研室,200032 [2]南京炼油厂职工医院职业病防治所

出　　处：《中华预防医学杂志》2000年第4期221-223,共3页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金!资助 (39670623)

摘　　要：目的　探讨汽油添加剂甲基叔丁基醚 (MTBE)对细胞周期和细胞凋亡的影响。方法采用流式细胞仪检测MTBE对NIH/3T3细胞周期 (染毒浓度分别为 1、2、4μl/ml,染毒时间为 2 4h)的影响 ;应用结晶紫染色法 ,通过检测Hela细胞的存活情况 ,了解MTBE对细胞凋亡的影响。结果　流式细胞仪结果显示 ,MTBE可使NIH/3T3细胞周期发生改变。表现为S期细胞减少 ,而处于G2 +M期的细胞增多 ,提示MTBE可能具有影响NIH/3T3细胞周期的作用 ,可诱导细胞增殖。这种作用持续存在于染毒后 48h的细胞中 ,到染毒后 96h ,细胞周期基本恢复正常。结晶紫染色法通过检测Hela细胞的存活情况 ,发现MTBE可明显拮抗肿瘤坏死因子 (TNF)α和放线菌酮所诱导的Hela细胞的凋亡。结论　MTBE可诱导细胞增殖和抑制细胞凋亡 ,这是其动物致癌性的可能机制之一。Objective To explore the effects of the new gasoline additive, methyl tertiary butyl ether (MTBE) on cell cycle and cell apoptosis. Methods Flow cytometry was used to evaluate the effect of MTBE (1, 2, 4 μl/ml, 24 h) on NIH/3T3 cell cycles; and the effect of MTBE on Hela cell apoptosis was evaluated by detecting cell survival using crystal violet staining. Results Flow cytometry showed that MTBE could change NIH/3T3 cell cycles, decrease the number of cells in S stage, and arrest cells at G 2+M stage. The results suggested that MTBE could affect NIH/3T3 cell cycles and induce cell proliferation. This situation existed 48 hours after the treatment, and cell cycles came back normal 96 hours after the treatment. By detecting cell survival using crystal violet staining, we found that MTBE could inhibit the apoptosis of Hela cells which was induced by tumor necrosis factor (TNF)α and cycloheximide. Conclusion MTBE′s carcinogenicity to animals may relate to induction of cell proliferation and inhibition of cell apoptosis.

关 键 词：细胞周期 甲基叔丁基醚 细胞凋亡 致癌性 

分 类 号：R135[医药卫生—劳动卫生]