玻璃红鲤MHCⅡ类基因多态性与组织表达分析  

Polymorphism and tissue expression analysis of MHC class Ⅱ genes in Cyprinus carpio var.wananensis

作　　者：张天睿[1] 刘至治[1] 刘俊[1] 赵雪锦[1] 王成辉[2] 

机构地区：[1]上海海洋大学鱼类研究室水产种质资源发掘与利用教育部重点实验室,上海201306 [2]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306

出　　处：《淡水渔业》2013年第3期15-20,共6页Freshwater Fisheries

基　　金：农业部公益性行业科研专项(200903045);上海市教育委员会科研创新基金(11YZ152);上海市水生生物学重点学科建设项目(S30701)

摘　　要：以cDNA为模板进行PCR扩增和克隆分析,从7尾玻璃红鲤(Cyprinus carpio var.wananensis)的70个有效克隆中,获得长度为624 bp的MHC IIα/β不同编码序列25/31个,分属于7/10个不同等位基因,揭示玻璃红鲤MHC II类基因的多态性仍较丰富。其中Cyca-DXA17*01和Cyca-DAB1*1201、Cyca-DAB1*1301、Cyca-DAB3*1901、Cyca-DAB3*2001、Cyca-DAB3*2101、Cyca-DAB3*2201为新发现的7个基因。分析表明,α1/β1结构域的核苷酸/氨基酸变异位点数明显高于α2/β2结构域;α1、β1结构域抗原结合位点(PBR)的ω值(ω=dN/dS,5.209和1.703)都大于1,揭示玻璃红鲤MHC II类基因在进化过程中受到正向选择作用。半定量分析显示,玻璃红鲤MHC II类基因在肾和脾中有较强表达,中等程度表达于胃、肝和肠,而鳃、心和眼的表达较弱。Based on cDNA templates,PCR amplifying and clone analysis were conducted on 624bp-length nucleotide of MHC class Ⅱ genes in Cyprinus carpio var.wananensis.25 and 31 different encoding sequences of MHC Ⅱ α and β genes were respectively identified from 70 clones of 7 individuals.These sequences were classified into 7 and 10 alleles.The results revealed relatively high polymorphism of MHC class Ⅱ genes in C.carpio var.wananensis.The seven alleles,Cyca-DXA17＊01,Cyca-DAB1＊1201,Cyca-DAB1＊1301,Cyca-DAB3＊1901,Cyca-DAB3＊2001,Cyca-DAB3＊2101,and Cyca-DAB3＊2201,were reported for the first time.The relative number of nucleotide or amino acid variable sites in α1 or β1 domain was higher than that in α2 or β2 domain.Especially,the ω values（ω=dN/dS） in peptide binding residues（PBR） of α1 and β1 domain were both above 1,implying positive selection pressure exerted on the MHC class Ⅱ genes of C.carpio var.wananensis.The MHC class Ⅱ transcript was detected at a high level in kidney and spleen,at a moderate level in stomach,liver and intestine,and at a low level in gills,heart and eyes by semi-quantitative PCR analysis.

关 键 词：玻璃红鲤(Cyprinus carpio var wananensis) 主要组织相容性复合体(MHC) 多态性 组织特异性表达 

分 类 号：S917.4[农业科学—水产科学]