应用多重RT-PCR检测甘肃‘成县迟蒜’中的大蒜潜隐病毒和洋葱黄矮病毒  

Detection of garlic latent virus and onion yellow dwarf virus in 'Chengxianchisuan' of Gansu by multiplex RT-PCR

作　　者：许蕊[1] 栗孟飞[1] 王雅琳[1] 李唯[1] 

机构地区：[1]甘肃省干旱生境作物学重点实验室,甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2013年第2期46-49,54,共5页Journal of Gansu Agricultural University

基　　金：甘肃省农牧厅生物技术研究项目(GNSW-2009-07)

摘　　要：根据大蒜潜隐病毒(garlic latent virus,GLV)和洋葱黄矮病毒(onion yellow dwarf virus,OYDV)的外壳蛋白基因核苷酸序列分别设计2对特异性引物,在单重RT-PCR检测的基础上,建立同时检测GLV和OYDV的多重RT-PCR技术体系.结果表明:该方法可从带病的甘肃‘成县迟蒜’大蒜样品中扩增出GLV(849bp)和OYDV(571bp)的2条特异性片段.GLV扩增产物与GenBank中登录的其他分离物核苷酸同源性在90.00%～99.76%,OYDV扩增产物与其他分离物核苷酸的同源性为90.00%～98.00%.Tow pairs of specific primer were designed based on the nucleotide sequence of garlic latent virus（GLV） and onion yellow dwarf virus（OYDV） coat protein region.A multiplex RT-PCR system was established to simultaneously detect two viruses of garlic,according to the result of single RT-PCR.All the samples from chengxianchisuan of Gansu infected by GLV and OYDV could be amplified by the multiplex RT-PCR,and yielding two specific bands of GLV（849 bp）,OYDV（571 bp）.Sequence analysis of the amplified products showed that the nucleotide sequences homology of GLV and OYDV was 90%~99.76% and 90%~98%,respectively compared with the sequences of other isolates in the GenBank..

关 键 词：大蒜 多重RT-PCR 大蒜潜隐病毒 洋葱黄矮病毒 

分 类 号：Q789[生物学—分子生物学]