球孢白僵菌菌株Bb10716对玉米象成虫的毒力测定  

Virulence of the Strain Bb10716 of Beauveria bassiana against the Adult of Sitophilus zeamais

作　　者：和晓敏[1] 杜广祖[1] 沙青[1] 肖关丽[2] 李正跃[1] 陈斌[1] 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201 [2]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2013年第3期293-297,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家基础研究计划(973)项目(2011CB100404);国家自然科学基金项目(30860005);云南省自然科学基金项目(2008CD124)

摘　　要：为了明确球孢白僵菌对玉米象的毒力,为玉米象生物防治提供依据,本研究采用浸渍法生物测定了球孢白僵菌Bb10716菌株在30,27,24,21,18,15℃下对玉米象Sitophilus zeamais(Coleoptera:Curculionidae)成虫的毒力。结果表明,在30,27,24,21,18,15℃条件下,接种Bb10716菌株1.76×108～1.76×103孢子/mL孢子悬浮液后第12天时玉米象成虫的校正死亡率分别为(2.22±0.21)%～(61.11±2.47)%,(3.33±0.26)%～(96.67±2.45)%,(6.67±0.23)%～(98.76±0.45)%,(6.67±0.23)%～(98.86±0.65)%,(0～56.67±1.54)%,(0～46.67±2.45)%。在30,27,24,21,18,15℃下,菌株Bb10716对玉米象成虫的毒力回归方程分别是Y=1.716+0.442X(R2=0.93),Y=0.81+0.74X(R2=0.92),Y=0.73+0.84X(R2=0.87),Y=0.48+1.26X(R2=0.84),Y=1.15+0.38X(R2=0.86)和Y=1.32+0.50X(R2=0.94),对玉米象成虫的致死中浓度LC50分别是2.68×107,4.49×105,1.20×105,1.70×105,9.16×107,8.33×107孢子/mL。用1.72×108孢子/mL孢子悬浮液接种处理后,对玉米象成虫致死中时LT50分别为(8.1±0.25),(6.5±0.36),(7.5±0.54),(7.1±0.54),(7.8±0.48),(11.6±1.12)d。由此表明,球孢白僵菌Bb10716菌株对玉米象成虫具有较强的毒力,在21,24,27℃下毒力最高,Bb10716菌株可作为玉米象成虫生物制剂开发用菌株。To evaluate the virulence of Beauveria bassiana and provide the base for the biocontrol on Sitophilus zeamais, the virulence of the strain Bb10716 of Beauveria bassiana against the adult of S. ze amais under the temperature of 30, 27, 24, 21, 18 and 15℃ was bioassayed in the laboratory. Results showed that the strain Bb1023 was virulent against the adult of the S. zeamais. The corrected mortali- ties by the 12th day after the inoculation of 1.76 x l0s - 1.76 x 103 conidia/mL concentration of Bb1023 were （2.22 ±0.21）% - （61.11±2.47）%, （3.33±0.26）% - （96.67 ±2.45）%,（6.67±0.23）% - （98.76 ±0.45）%, （6.67 ±0.23）% - （98.86 ±0.65）%, （0-56.67_ 1.54 ） % , （0 - 46.67 ± 2. 45 ） % , respectively. The virulence regression equations under the tempera- ture of 30, 27, 24, 21, 18, 15 were Y=1.716 ＋0.442X （R2 =0.93）, Y=0.81 ＋0.74X （R2 = 0.92）, Y=0.73 ＋0.84X （R2 =0.87）, Y=0.48 ± 1.26X （R2 =0.84）, Y=l. 15 ＋0.38X （ R2 = 0. 86） and Y = 1.32 ＋ 0. 50X （R2 = 0.94）, respectively. The LCso values under the tempera- ture of 30, 27, 24, 21, 18, 15~C were 2.68 x10^7, 4.49 x10^5, 1.20 x10^5, 1.70x10^5, 9. 16 x 10^7, 8.33 x 10^7 conidia/rnL, respectively, and the LTs0 values were （8. 1±0. 25）, （6.5 ±0. 36）, （7.5±0.54）, （7.1±0.54）, （7.8±0.48） and （11.6±1.12） d. So that the strain of Bbl0716 of B. bassiana is virulent against the adult of S. zeamais and the highest virulence was under the temperature of 27, 24 and 21℃. The stain Bb10716 shows a potential for the development of biological control agent against the S. zeamais.

关 键 词：玉米象 球孢白僵菌 生物测定 毒力 时间—剂量—死亡率 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]