猪—猴异种细胞移植治疗非人类灵长类糖尿病的应用研究  

Study on Application of Pig-rhesus Cell Xenograft to Treat the Non-human Primates of Diabetes

作　　者：李汝红[1,2] 李鹏[1] 于海东[1] 侯宗柳[3] 和占龙[4] 王殿华[2] 

机构地区：[1]昆明医科大学附属延安医院普外一科,云南昆明650051 [2]昆明医科大学药学院,云南昆明650500 [3]昆明医科大学附属延安医院中心实验室,云南昆明650051 [4]中国医学科学院生物学研究所,云南昆明650118

出　　处：《云南农业大学学报（自然科学版）》2013年第3期317-321,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省重大攻关项目(2009CA010);云南省科技厅-昆医联合专项(2012FB101)

摘　　要：为探讨滇南小耳猪胰岛细胞对恒河猴糖尿病的治疗作用,将滇南小耳猪胰岛细胞均按照90 000 IEQ/kg猴体重移植给糖尿病猴(n=6),检测移植后猪C肽水平和猴C肽、血糖、糖化血红蛋白等指标,分析不同时间糖耐量实验的反应程度及对受体猴血生化、血常规等指标的变化。结果表明:5只猴移植成功,移植成功率83.33%;移植后第4周猴血糖水平开始下降,第6周基本恢复到正常水平,持续到22周;外源性胰岛素用量与血糖水平变化呈正比,在第6～22周时开始几乎脱离外源性胰岛素的治疗。移植前后对肝肾功能无影响。表明滇南小耳猪胰岛细胞对非人类灵长类恒河猴糖尿病有治疗作用;移植失败仅为移植物失活,对猴重要器官结构和功能无明显损害。To explore the effect of xenograft islet cell from Diannan small-ear pig for the treatment of monkey diabetes models, the islet cells from Diannan small-ear pig were planted to diabetic monkey with the dosage of 90 000 IEQ/kg. The levels of C peptide of pig and monkey, blood glucose level and glycosylated hemoglobin level were assayed. The oral glucose tolerance test in different time points, biochemical index and blood routine examination were also included in our study. The results showed that the transplantation of the islet cells were achieved in five monkey models with the success rate of 83.33%. The blood glucose level of the monkey decreased since 4 weeks after the transplantation andrecovered to the normal level at the 6th week. The glucose level had maintained normal for 22 weeks. The dosage of the insulin administration was positively correlated with changes of the blood glucose lev- el. The monkey could live with normal blood glucose level nearly without insulin administration. Mo- reover, the transplantation of the islet cell has no side-effect on the liver and kidney function of the monkey model. It is concluded that the islet cell from Diannan small-ear pig transplantation is effective to treat the non-primate rhesus monkey model of diabetes. The failure of this treatment is only seen in one case, which is attributed to the inactivation of the graft. The transplantation of the islet cell has no side-effect on the function of liver and kidney.

关 键 词：胰岛移植 猪胰岛细胞 恒河猴 C肽 血糖 

分 类 号：R617[医药卫生—外科学]