温度和pH对农杆菌介导转化橡胶树悬浮细胞的影响  

Effects of Co-culture Medium pH and Temperature on Agrobacterium-mediated Transformation of Hevea brasiliensis Embryogenic Cell Suspension

作　　者：周权男[1] 谢黎黎[1] 黄天带[1] 华玉伟[1] 孙爱花[1] 黄华孙[1] 

机构地区：[1]中国热带农业科学院橡胶研究所,国家橡胶树育种中心,海南儋州571737

出　　处：《西北林学院学报》2013年第3期131-133,209,共4页Journal of Northwest Forestry University

基　　金：中央级公益性科研院所基本科研业务费中国热带农业科学院橡胶研究所基本科研业务费专项(1630022011005);现代农业产业技术体系建设专项(NYCYTX-34)

摘　　要：橡胶树胚性细胞悬浮培养系及其植株再生体系的建立可为橡胶树遗传转化研究提供良好的材料。以橡胶树品种热研8-79悬浮培养的胚性细胞为受体,研究了共培养基pH值和共培养温度对农杆菌介导的橡胶树胚性悬浮细胞遗传转化的影响。结果表明:pH5.2～5.4、20℃～22℃共培养适宜于农杆菌介导的橡胶树胚性悬浮细胞的遗传转化。The establishment of embryogenic cell suspension culture system and its regeneration system of Hevea brasiliensis can provide sufficient and fine materials for studying genetic transformation.In this study,embryogenic suspension cells of rubber tree clone Reyan 8-79 were used as recipients.Factors influencing genetic transformation such as pH value of co-culture medium and temperature were studied.The optimal pH and temperature were 5.2 to 5.4,and 20 to 22℃.

关 键 词：橡胶树 胚性细胞 悬浮培养 遗传转化 温度 PH 

分 类 号：S722.3[农业科学—林木遗传育种]